Molecular epidemiology of malaria in Cameroon. XI. Geographic distribution of Plasmodium falciparum isolates with dihydrofolate reductase gene mutations in southern and central Cameroon.

Leonardo K Basco Laboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC) and Unité de Recherche Paludologie Afro-tropicale, Institut de Recherche pour le Développement (IRD), Yaounde, Cameroon.

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Mathieu Ndounga Laboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC) and Unité de Recherche Paludologie Afro-tropicale, Institut de Recherche pour le Développement (IRD), Yaounde, Cameroon.

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Mathurin Tejiokem Laboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC) and Unité de Recherche Paludologie Afro-tropicale, Institut de Recherche pour le Développement (IRD), Yaounde, Cameroon.

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Vincent Foumane Ngane Laboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC) and Unité de Recherche Paludologie Afro-tropicale, Institut de Recherche pour le Développement (IRD), Yaounde, Cameroon.

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Jean-Christian Youmba Laboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC) and Unité de Recherche Paludologie Afro-tropicale, Institut de Recherche pour le Développement (IRD), Yaounde, Cameroon.

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Pascal Ringwald Laboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC) and Unité de Recherche Paludologie Afro-tropicale, Institut de Recherche pour le Développement (IRD), Yaounde, Cameroon.

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Georges Soula Laboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC) and Unité de Recherche Paludologie Afro-tropicale, Institut de Recherche pour le Développement (IRD), Yaounde, Cameroon.

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DOI:
https://doi.org/10.4269/ajtmh.2002.67.378
Volume/Issue:
Volume 67: Issue 4
Page(s):
378–382
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The DNA sequence of the dihydrofolate reductase (dhfr) gene, a molecular marker for pyrimethamine resistance, was determined for 178 field isolates of Plasmodium falciparum collected along the east-west axis in southern Cameroon. The proportion of isolates having the wild-type dhfr allele varied from 48.1% in the east (city of Bertoua) to 11.3-15.7% in central provinces (Yaounde and Eseka) and 0% in the littoral region (port city of Douala). Isolates with a single Asn-108 mutation or double mutations (Ile-51 or Arg-59 and Asn-108) constituted approximately 10% of the samples. Isolates with triple mutations (Ile-51, Arg-59, and Asn-108) were present in an equal proportion (48.1%) as the wild-type isolates in the east (Bertoua), while triple mutations predominated in Yaounde (62.3%), Eseka (62.7%), and Douala (78.9%). The distribution of triple dhfr mutations along the east-west axis in southern Cameroon suggests the presence of a decreasing gradient from the west coastal region to the central region and then to the east towards the interior of the country.

Published Online:
Oct 2002
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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