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Genetic variation within the premembrane coding region of dengue viruses from the Yucatan peninsula of Mexico.
Francisco J DÃaz
Francisco J DÃaz
Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
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Jose A Farfán-Ale
Jose A Farfán-Ale
Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
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Ken E Olson
Ken E Olson
Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
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MarÃa A Loroño-Pino
MarÃa A Loroño-Pino
Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
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Duane J Gubler
Duane J Gubler
Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
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Carol D Blair
Carol D Blair
Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
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William C Black 4th
William C Black 4th
Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
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Barry J Beaty
Barry J Beaty
Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
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Single-strand conformation polymorphism (SSCP) and sequence analyses were used to characterize genetic polymorphisms and phylogenetic relationships, respectively, among dengue (DEN) viruses isolated between 1980 and 1997 from Yucatan, Mexico and surrounding states. Amplified cDNAs from the premembrane (prM) coding region of the DEN viruses were characterized by SSCP. There were six distinct haplotypes of DEN-1 viruses, four haplotypes of DEN-2, four haplotypes of DEN-3, and eight haplotypes of DEN-4. The diversity index for DEN-3 isolates was significantly lower than that of the other serotypes, probably reflecting the recent introduction of this viral serotype into Mexico. The SSCP was a sensitive (84.5%) and specific (95.5%) technique for identifying nucleotide substitutions. Sequence analyses provided insight into the phylogenetic relationships of the DEN strains isolated in Yucatan. One DEN-2 isolate from 1996 was demonstrated to cluster with viruses of the Sri Lanka genotype, none of which have been detected before in the Americas.
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