A putative receptor for dengue virus in mosquito tissues: localization of a 45-kDa glycoprotein.

María Yazi Mendoza Departamento de Patologia Experimental, Centro de Investigación y de Estudios Avanzados del IPN, México City, México.

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Juan Santiago Salas-Benito Departamento de Patologia Experimental, Centro de Investigación y de Estudios Avanzados del IPN, México City, México.

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Humberto Lanz-Mendoza Departamento de Patologia Experimental, Centro de Investigación y de Estudios Avanzados del IPN, México City, México.

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Salvador Hernández-Martínez Departamento de Patologia Experimental, Centro de Investigación y de Estudios Avanzados del IPN, México City, México.

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Rosa M del Angel Departamento de Patologia Experimental, Centro de Investigación y de Estudios Avanzados del IPN, México City, México.

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DOI:
https://doi.org/10.4269/ajtmh.2002.67.76
Volume/Issue:
Volume 67: Issue 1
Page(s):
76–84
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Dengue virus (DENV) infects target cells by attaching to various cell receptors, many of which are still unknown. In C6/36 cells (Aedes albopictus cell line), DENV-4 bound to two glycoproteins of 40 and 45 kDa, located on the cell surface. Preincubation of cells with polyclonal antibody against the 45-kDa protein specifically blocked DENV-4 infection of C6/36 cells. The antibody and purified DENV-4 detected the 45-kDa molecule in total extracts from eggs, larvae, and pupae as well as from the midgut, ovary, and salivary glands from adult-stage Aedes aegypti mosquitoes, whereas in malphigian tubules it was absent. This suggests that the distribution of the 45-kDa protein correlates with tissue tropism of DENV infection in mosquitoes. The 45-kDa molecule was not detected in Anopheles albimanus mosquito. The relevance of our findings is discussed from the pathogenetic and vector competence viewpoints.

Author Notes

Published Online:
Jul 2002
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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