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Genetic complexity of Plasmodium falciparum gametocytes isolated from the peripheral blood of treated Gambian children.
Colin J Sutherland
Colin J Sutherland
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk
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Ali Alloueche
Ali Alloueche
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk
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Louisa McRobert
Louisa McRobert
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk
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Rosalynn Ord
Rosalynn Ord
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk
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Jamie Leggat
Jamie Leggat
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk
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Georges Snounou
Georges Snounou
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk
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Margaret Pinder
Margaret Pinder
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk
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Geoffrey A T Targett
Geoffrey A T Targett
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom. colin.sutherland@lshtm.ac.uk
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The genetic complexity of Plasmodium falciparum gametocytes isolated from Gambian children participating in a controlled trial of anti-malarial therapy was investigated. RNA and DNA were prepared from gametocyte-positive blood, which was also used in transmission experiments with Anopheles gambiae mosquitoes. Amplification by a reverse transcriptase-polymerase chain reaction (RT-PCR) of transcripts from the genes for the ring-infected erythrocyte surface antigen and the 16-kD antigen, which exhibit asexual and sexual stage-specific expression, was used to identify 30 post-treatment gametocyte isolates in which trophozoites persisted below the threshold of detection by microscopy. These included isolates from children who received sulfadoxine/pyrimethamine plus artesunate. Twenty-nine gametocyte-positive isolates that were free of subpatent trophozoites were examined further by PCR amplification of polymorphic genomic loci. We estimate that an average minimum of 2.3 genotypes occurred in these gametocyte-only isolates, and many of these were shown to be infective to mosquitoes. Thus, meiotic recombination between different genotypes is predicted to be a common event in this study area.
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| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 412 | 375 | 153 |
| Full Text Views | 415 | 6 | 1 |
| PDF Downloads | 68 | 8 | 1 |