Evaluation of antigens from various Leishmania species in a Western blot for diagnosis of American tegumentary leishmaniasis.

Cely Cristina Martins Gonçalves Laboratório de Tripanosomatídeos, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.

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Edna Maria Vissoci Reiche Laboratório de Tripanosomatídeos, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.

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Benício Alves De Abreu Filho Laboratório de Tripanosomatídeos, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.

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Thais Gomes Verzignassi Silveira Laboratório de Tripanosomatídeos, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.

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Tânia Cristina Felizardo Laboratório de Tripanosomatídeos, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.

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Karoline Rocha Maia Laboratório de Tripanosomatídeos, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.

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Rafael Costacurta Laboratório de Tripanosomatídeos, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.

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Evandro José Padovesi Laboratório de Tripanosomatídeos, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.

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Benedito Prado Dias Filho Laboratório de Tripanosomatídeos, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.

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Shiduca Itow Jankevicius Laboratório de Tripanosomatídeos, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.

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José Vitor Jankevicius Laboratório de Tripanosomatídeos, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.

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DOI:
https://doi.org/10.4269/ajtmh.2002.66.91
Volume/Issue:
Volume 66: Issue 1
Page(s):
91–102
Restricted access
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A Western blot method that uses antigens from culture promastigote forms of Leishmania (Viannia) braziliensis, L. (Leishmania) amazonensis, L. (Leishmania) tropica, and a trypanosomatid (strain 268T) isolated from naturally infected tomatoes was evaluated for laboratory diagnosis of American tegumentary leishmaniasis (ATL). Serum samples were obtained from 108 patients with ATL (group I), 23 chagasic patients (group II), 32 patients with other diseases (group III), and 78 healthy individuals (group IV). The overall analysis showed a sensitivity of 76.90%, 90.40%, 78.50%, and 87.90%, a specificity of 100%, 93.80%, 87.80%, and 77.10%, a positive predictive value of 100%, 94.00%, 89.50%, and 72.50%, a negative predictive value of 75.70%, 90.00%, 75.40%, and 90.20%, and a concordance coefficient kappa of 0.7358, 0.8400, 0.6491, and 0.6287 for L. (V.) braziliensis, L. (L.) amazonensis, L. (L.) tropica, and strain 268T antigens, respectively. The antigenic profile recognized by serum samples from patients with ATL and with Chagas' disease permits serologic distinction between these infections.

Author Notes

Published Online:
Jan 2002
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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