Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
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0002-9637
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Speciation of human microsporidia by polymerase chain reaction single-strand conformation polymorphism.

D P FedorkoMicrobiology Service and Chemistry Service, Department of Laboratory Medicine, Warren C. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA. dfedorko@nih.gov

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N A NelsonMicrobiology Service and Chemistry Service, Department of Laboratory Medicine, Warren C. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA. dfedorko@nih.gov

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E S DidierMicrobiology Service and Chemistry Service, Department of Laboratory Medicine, Warren C. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA. dfedorko@nih.gov

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D BertucciMicrobiology Service and Chemistry Service, Department of Laboratory Medicine, Warren C. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA. dfedorko@nih.gov

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R M DelgadoMicrobiology Service and Chemistry Service, Department of Laboratory Medicine, Warren C. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA. dfedorko@nih.gov

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A M HruszkewyczMicrobiology Service and Chemistry Service, Department of Laboratory Medicine, Warren C. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA. dfedorko@nih.gov

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DOI:
https://doi.org/10.4269/ajtmh.2001.65.397
Volume/Issue:
Volume 65: Issue 4
Page(s):
397–401
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We describe the application of single-strand conformation polymorphism (SSCP) analysis to the speciation of human microsporidia after polymerase chain reaction (PCR) amplification with the panmicrosporidian primers PMP1 and PMP2. We compared the DNA extracted and amplified from different genotypes or isolates of Enterocytozoon bieneusi, Encephalitozoon cuniculi, E. hellem, and E. intestinalis plus an isolate of Vittaforma corneae. The PCR-SSCP, when performed at 20 degrees C, generated 2 bands in distinctive, reproducible patterns in polyacrylamide gels for each species of microsporidia tested, regardless of genotype or isolate. We found PCR-SSCP to be an easy and reproducible method for speciation of human microsporidia when the primer pair PMP1 and PMP2 is used.

Published Online:
Oct 2001
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