Past two years Past Year Past 30 Days
Abstract Views 2 2 2
Full Text Views 228 191 2
PDF Downloads 40 31 2
 
 
 
 
 
 
 
 
 
 
 

Short report: genetic heterogeneity of Japanese encephalitis virus assessed via analysis of the full-length genome sequence of a Korean isolate.

J H NamDepartment of Virology, Korea National Institute of Health, Seoul.

Search for other papers by J H Nam in
Current site
Google Scholar
PubMed
Close
,
S L ChaeDepartment of Virology, Korea National Institute of Health, Seoul.

Search for other papers by S L Chae in
Current site
Google Scholar
PubMed
Close
,
S Y WonDepartment of Virology, Korea National Institute of Health, Seoul.

Search for other papers by S Y Won in
Current site
Google Scholar
PubMed
Close
,
E J KimDepartment of Virology, Korea National Institute of Health, Seoul.

Search for other papers by E J Kim in
Current site
Google Scholar
PubMed
Close
,
K S YoonDepartment of Virology, Korea National Institute of Health, Seoul.

Search for other papers by K S Yoon in
Current site
Google Scholar
PubMed
Close
,
B I KimDepartment of Virology, Korea National Institute of Health, Seoul.

Search for other papers by B I Kim in
Current site
Google Scholar
PubMed
Close
,
Y S JeongDepartment of Virology, Korea National Institute of Health, Seoul.

Search for other papers by Y S Jeong in
Current site
Google Scholar
PubMed
Close
, and
H W ChoDepartment of Virology, Korea National Institute of Health, Seoul.

Search for other papers by H W Cho in
Current site
Google Scholar
PubMed
Close
View More View Less
Restricted access

We determined the full-length genome sequence of Japanese encephalitis virus (JEV) K94P05 isolated in Korea. Sequence analysis showed that the 10,963-nucleotide-long RNA genome of K94P05 was 13 or 14 nucleotides shorter than the genome of other JEV isolates because of a deletion in the 3' noncoding region of K94P05. Compared with sequences of other JEV isolates, the full-length nucleotide sequence showed 89.0-89.6% homology, and the deduced amino acid sequence showed between 96.4-97.3% homology. A region of approximately 60 nucleotides immediately downstream of the open reading frame stop codon of K94P05 showed high sequence variability as compared with other JEV isolates. K94P05 formed a distinct group within a phylogenetic tree established with the full-length genome sequences. Cross-neutralization studies showed that polyclonal antibodies to Korean isolates were 3 times better at neutralizing the Korean isolates than antibodies to Nakayama-NIH. These findings suggest that Korean JEV K94P05 is genetically and antigenically distinct from other Asian JEV isolates.

Save