Short report: A practical technique for the cryopreservation of Dirofilaria immitis, Brugia malayi, and Wuchereria bancrofti microfilariae.

L C Bartholomay Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, 53706-1581, USA. bartholomay@ahabs.wisc.edu

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H A Farid Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, 53706-1581, USA. bartholomay@ahabs.wisc.edu

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E El Kordy Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, 53706-1581, USA. bartholomay@ahabs.wisc.edu

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B M Christensen Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, 53706-1581, USA. bartholomay@ahabs.wisc.edu

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DOI:
https://doi.org/10.4269/ajtmh.2001.65.162
Volume/Issue:
Volume 65: Issue 2
Page(s):
162–163
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A technique to cryopreserve microfilariae has been developed. This method was used to cryopreserve microfilariae of Dirofilaria immitis, Brugia malayi, and Wuchereria bancrofti at a controlled rate of 1 degree C/min by use of a freezing tank. Microfilariae of each of these species retained their ability to infect susceptible mosquito species and develop to the infective stage after cryopreservation. The method presented here is quickly and easily carried out with inexpensive equipment.

Author Notes

Published Online:
Aug 2001
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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