Identification of frequently recognized dimorphic T-cell epitopes in plasmodium falciparum merozoite surface protein-1 in West and East Africans: lack of correlation of immune recognition and allelic prevalence.

E A Lee Institute of Molecular Medicine, Nuffield Department Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom. elee@molbiol.ox.ac.uk

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K L Flanagan Institute of Molecular Medicine, Nuffield Department Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom. elee@molbiol.ox.ac.uk

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K Odhiambo Institute of Molecular Medicine, Nuffield Department Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom. elee@molbiol.ox.ac.uk

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W H Reece Institute of Molecular Medicine, Nuffield Department Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom. elee@molbiol.ox.ac.uk

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C Potter Institute of Molecular Medicine, Nuffield Department Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom. elee@molbiol.ox.ac.uk

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R Bailey Institute of Molecular Medicine, Nuffield Department Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom. elee@molbiol.ox.ac.uk

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K Marsh Institute of Molecular Medicine, Nuffield Department Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom. elee@molbiol.ox.ac.uk

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M Pinder Institute of Molecular Medicine, Nuffield Department Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom. elee@molbiol.ox.ac.uk

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A V Hill Institute of Molecular Medicine, Nuffield Department Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom. elee@molbiol.ox.ac.uk

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M Plebanski Institute of Molecular Medicine, Nuffield Department Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom. elee@molbiol.ox.ac.uk

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DOI:
https://doi.org/10.4269/ajtmh.2001.64.194
Volume/Issue:
Volume 64: Issue 3
Page(s):
194–203
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The merozoite surface protein-1 (MSP1) is the most studied malaria blood-stage vaccine candidate. Lymphokines such as interferon gamma (IFN-gamma) and interleukin 4 (IL-4) may mediate blood-stage specific protection. Here we identify Plasmodiumfalciparum MSP1 T-cell epitopes capable of rapid induction of IFN-gamma and/or IL-4 from peripheral blood mononuclear cells of East and West African donors. Both allelic forms of these novel MSP1 T-cell epitopes were stimulatory. An unusually high numbers of Gambian responders (> 80%) to these epitopes were observed, suggesting that MSPI reactivity may have been underestimated previously in this population. Surprisingly, IFN-gamma responses to allelic T-cell epitopes failed to correlate with differential antigenic exposure in The Gambia compared to Kenya. These results suggest an unexpected level of immunoregulation of IFN-gamma response with variable allelic T-cell reactivity independent of the level of antigenic exposure. Further analysis of the mechanisms determining this response pattern may be required if vaccines are to overcome this allelic reactivity bias in malaria-exposed populations.

Author Notes

Published Online:
Mar 2001
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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