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Molecular typing of Trichomonas vaginalis isolates by HSP70 restriction fragment length polymorphism.
J K Stiles
J K Stiles
Department of Medicine, University of Mississippi Medical Center, Jackson 39216-4505, USA.
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P H Shah
P H Shah
Department of Medicine, University of Mississippi Medical Center, Jackson 39216-4505, USA.
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L Xue
L Xue
Department of Medicine, University of Mississippi Medical Center, Jackson 39216-4505, USA.
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J C Meade
J C Meade
Department of Medicine, University of Mississippi Medical Center, Jackson 39216-4505, USA.
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W B Lushbaugh
W B Lushbaugh
Department of Medicine, University of Mississippi Medical Center, Jackson 39216-4505, USA.
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J D Cleary
J D Cleary
Department of Medicine, University of Mississippi Medical Center, Jackson 39216-4505, USA.
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R W Finley
R W Finley
Department of Medicine, University of Mississippi Medical Center, Jackson 39216-4505, USA.
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Subtyping isolates of Trichomonas vaginalis is an essential tool for understanding the epidemiology of this common sexually-transmitted disease. Restriction fragment length polymorphism (RFLP) analysis employing a probe from the heat-inducible cytoplasmic HSP70 gene family hybridized with EcoR I-digested genomic DNA was used in the molecular typing of Trichomonas isolates. Analysis of five American Type Culture Collection (ATCC) reference strains and 31 Jackson, Mississippi, isolates from six male and 21 female patients, revealed 10 distinct RFLP pattern subtypes of Trichomonas. The subtypes were temporally stable and cosmopolitan. The RFLP profiles seen in Maryland, Ohio, Massachusetts, and New York ATCC strains were identical to those of some Mississippi isolates, even though the samples were isolated 10-35 years apart. There was no correlation between metronidazole resistance and RFLP subtype with resistant isolates from eight patients distributed among six different subtypes.
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| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 864 | 811 | 66 |
| Full Text Views | 607 | 10 | 1 |
| PDF Downloads | 82 | 10 | 0 |