The development of an immunoassay for the detection of artemisinin compounds in urine.

T A Eggelte Department of Clinical Pharmacology and Pharmacotherapy, Academic Medical Centre, Amsterdam, The Netherlands.

Search for other papers by T A Eggelte in
Current site
Google Scholar
PubMed Close
,
M A van Agtmael Department of Clinical Pharmacology and Pharmacotherapy, Academic Medical Centre, Amsterdam, The Netherlands.

Search for other papers by M A van Agtmael in
Current site
Google Scholar
PubMed Close
,
T D Vuong Department of Clinical Pharmacology and Pharmacotherapy, Academic Medical Centre, Amsterdam, The Netherlands.

Search for other papers by T D Vuong in
Current site
Google Scholar
PubMed Close
, and
C J van Boxtel Department of Clinical Pharmacology and Pharmacotherapy, Academic Medical Centre, Amsterdam, The Netherlands.

Search for other papers by C J van Boxtel in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.4269/ajtmh.1999.61.449
Volume/Issue:
Volume 61: Issue 3
Page(s):
449–456
Restricted access
Get Permissions

We have produced monoclonal antibodies against artelinic acid and investigated the reactivity with artemisinin drugs and metabolites. Antibody F170-10 is fairly specific for artelinic acid but does bind artemisinin and artemether (3-5% cross-reactivity). Dihydroartemisinin, artesunate, and metabolites of artemisinin showed less reactivity. With this antibody, an inhibition ELISA has been set up to detect artemisinin compounds in urine. In healthy subjects who received a single oral dose of artemisinin, artemether, artesunate or dihydroartemisinin, ELISA reactivity in urine was found. This reactivity in urine paralleled the plasma concentrations of artemether and dihydroartemisinin. The results show that this immunoassay for artelinic acid can be used to detect artemisinin compounds in urine for about 8 hr after intake. With a more sensitive test, this simple method as a urine dipstick may be become useful for drug use and compliance studies in malaria-endemic areas where the artemisinin derivatives are increasingly used.

Author Notes

Published Online:
Sep 1999
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Past two years Past Year Past 30 Days
Abstract Views 399 246 13
Full Text Views 243 1 0
PDF Downloads 35 2 0
 

 

 

 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save