Immunochemical characterization and diagnostic potential of a 63-kilodalton Schistosoma antigen.

A M AttallahBiotechnology Research Center, New Damietta City, Egypt.

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E YonesBiotechnology Research Center, New Damietta City, Egypt.

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H IsmailBiotechnology Research Center, New Damietta City, Egypt.

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S A El MasryBiotechnology Research Center, New Damietta City, Egypt.

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A TabllBiotechnology Research Center, New Damietta City, Egypt.

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A A EleneinBiotechnology Research Center, New Damietta City, Egypt.

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N A El GhawalbyBiotechnology Research Center, New Damietta City, Egypt.

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Schistosoma circulating antigens were used for the detection of active infection. Anti-S. mansoni IgG2a monoclonal antibody (MAb) designated C5C4 was generated. The target epitope of this MAb was detected in adult worms, eggs, and cercariae antigenic extracts of S. mansoni and S. haematobium, had a molecular size of 63 kD, and was not detected in Fasciola hepatica and Ascaris. In addition, a 50-kD degradation product was identified only in the urine of infected individuals. Analysis by high-performance liquid chromatography of the purified antigen demonstrated only one peak. The 63-kD antigen was characterized as a protein containing 40.4% hydrophobic, 7.5% acidic, and 8.8% basic amino acids. The C5C4 MAb was used in a Fast Dot-ELISA for rapid and simple diagnosis of human schistosomiasis. The 63-kD circulating antigen was detected in 92% of urine samples from 330 S. mansoni-infected individuals, with 16% false-positive results among 130 noninfected individuals.

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