A semisynthetic fetal calf serum-free liquid medium for in vitro cultivation of Leishmania promastigotes.

S A AliInstitute of Biochemistry, and Department of Pharmacy, University of Balochistan, Quetta, Pakistan.

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J IqbalInstitute of Biochemistry, and Department of Pharmacy, University of Balochistan, Quetta, Pakistan.

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B AhmadInstitute of Biochemistry, and Department of Pharmacy, University of Balochistan, Quetta, Pakistan.

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M MasoomInstitute of Biochemistry, and Department of Pharmacy, University of Balochistan, Quetta, Pakistan.

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A semisynthetic, autoclavable, liquid medium that does not require inclusion of fetal calf serum (FCS) for the successful cultivation of Leishmania promastigotes in vitro is described. The parasites were grown in commercially available medium 199 supplemented with 10% FCS and 2% mammalian urine and the growth was compared with the same medium without FCS. The growth of the parasites in the semidefined medium without FCS was found to be as good as or slightly better that with medium 199 supplemented with FCS.

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