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Short Report: Leishmania DNA in Phlebotomus and Sergentomyia Species during a Kala-Azar Epidemic
Sandeep Mukherjee
Sandeep Mukherjee
Indian Institute of Chemical Biology, Calcutta, India
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Mohammed Quamarul Hassan
Mohammed Quamarul Hassan
Indian Institute of Chemical Biology, Calcutta, India
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Anil Ghosh
Anil Ghosh
Indian Institute of Chemical Biology, Calcutta, India
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Kashi Nath Ghosh
Kashi Nath Ghosh
Indian Institute of Chemical Biology, Calcutta, India
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Amal Bhattacharya
Amal Bhattacharya
Indian Institute of Chemical Biology, Calcutta, India
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Samit Adhya
Samit Adhya
Indian Institute of Chemical Biology, Calcutta, India
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The presence of Leishmania donovani DNA in sand flies caught in Indian kala-azar patients' dwellings during the epidemic of 1990–1992 was studied using the polymerase chain reaction (PCR). Amplification of miniexon-derived RNA genes and gpG3 mRNA was achieved in single Phlebotomus argentipes, P. papatasi, and Sergentomyia babu flies. The data suggest the possible involvement of multiple sandfly species in kala-azar transmission.
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