Immunoblot with Cerebrospinal Fluid from Patients with Neurocysticercosis using Antigen from Cysticerci of Taenia Solium and Taenia Crassiceps

Adelaide J. VazFaculty of Pharmaceutical Sciences, and Faculty of Medicine, University of Sao Paulo, Adolfo Lutz Institute, Butantan Institute, Emilio Ribas Infectology Institute, Tropical Medicine Institute of Sao Paulo, Sao Paulo, Brazil

Search for other papers by Adelaide J. Vaz in
Current site
Google Scholar
PubMed
Close
,
Celia M. NunesFaculty of Pharmaceutical Sciences, and Faculty of Medicine, University of Sao Paulo, Adolfo Lutz Institute, Butantan Institute, Emilio Ribas Infectology Institute, Tropical Medicine Institute of Sao Paulo, Sao Paulo, Brazil

Search for other papers by Celia M. Nunes in
Current site
Google Scholar
PubMed
Close
,
Roxane M. F. PiazzaFaculty of Pharmaceutical Sciences, and Faculty of Medicine, University of Sao Paulo, Adolfo Lutz Institute, Butantan Institute, Emilio Ribas Infectology Institute, Tropical Medicine Institute of Sao Paulo, Sao Paulo, Brazil

Search for other papers by Roxane M. F. Piazza in
Current site
Google Scholar
PubMed
Close
,
Jose A. LivramentoFaculty of Pharmaceutical Sciences, and Faculty of Medicine, University of Sao Paulo, Adolfo Lutz Institute, Butantan Institute, Emilio Ribas Infectology Institute, Tropical Medicine Institute of Sao Paulo, Sao Paulo, Brazil

Search for other papers by Jose A. Livramento in
Current site
Google Scholar
PubMed
Close
,
Marcos V. Da SilvaFaculty of Pharmaceutical Sciences, and Faculty of Medicine, University of Sao Paulo, Adolfo Lutz Institute, Butantan Institute, Emilio Ribas Infectology Institute, Tropical Medicine Institute of Sao Paulo, Sao Paulo, Brazil

Search for other papers by Marcos V. Da Silva in
Current site
Google Scholar
PubMed
Close
,
Paulo M. NakamuraFaculty of Pharmaceutical Sciences, and Faculty of Medicine, University of Sao Paulo, Adolfo Lutz Institute, Butantan Institute, Emilio Ribas Infectology Institute, Tropical Medicine Institute of Sao Paulo, Sao Paulo, Brazil

Search for other papers by Paulo M. Nakamura in
Current site
Google Scholar
PubMed
Close
, and
A. Walter FerreiraFaculty of Pharmaceutical Sciences, and Faculty of Medicine, University of Sao Paulo, Adolfo Lutz Institute, Butantan Institute, Emilio Ribas Infectology Institute, Tropical Medicine Institute of Sao Paulo, Sao Paulo, Brazil

Search for other papers by A. Walter Ferreira in
Current site
Google Scholar
PubMed
Close
Restricted access

A comparative study was conducted on membrane (M) and vesicular fluid (VF) from cysticerci of Taenia solium (Tso) obtained from naturally infected swine and the Taenia crassiceps ORF strain (Tc) maintained by experimental infection of female BALB/c mice. The study was carried out using immunoblotting to detect antibodies in cerebrospinal fluid (CSF) from patients with neurocysticercosis. No reactivity was observed in the 32 samples from a control group. Of the 23 CSF fluid samples from patients with neurocysticercosis, 22 (95.6%) were reactive in the M-Tso blot and 21 (91.3%) were reactive in the other three blots (VF-Tso, M-Tc, and VF-Tc). Immunodominant peptides in each antigen were 98–92 kD, 56–52 kD, and 72–68 kD in M-Tso; 72–68 kD, 120 kD, 155 kD, 98–94 kD, 76 kD, and 115–108 kD in VF-Tso: 72 kD, 62 kD, and 42 kD in M-Tc; and 72–68 kD and 95-92 kD in VF-Tc. The cross-reactivity observed in the immunoblots performed on CSF samples from patients with neurocysticercosis indicates that the parasites share important epitopes present at sufficient concentrations for use in immunologic tests.

Save