Isolation and Characterization of Pirital Virus, a Newly Discovered South American Arenavirus

Charles F. Fulhorst Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Michael D. Bowen Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Rosa Alba Salas Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Nuris M. C. De Manzione Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Gloria Duno Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Antonio Utrera Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Thomas G. Ksiazek Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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C. J. Peters Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Stuart T. Nichol Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Edith De Miller Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Duilia Tovar Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Bricio Ramos Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Clovis Vasquez Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Robert B. Tesh Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Instituto Nacional de Higiene Rafael Rangel, Ciudad Universitaria, Ministerio de Sanidad y Asistenicia Social, Region Sanitaria del Estado Portuguesa, Universidad Nacional Experimental de Los Llanos Occidentales Ezequiel Zamora, Galveston, Texas, Georgia

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Specific rodent species are principal hosts for each of the well-characterized members of the virus family Arenaviridae. Guanarito virus (Arenaviridae) is the etiologic agent of Venezuelan hemorrhagic fever. A previous study on the epidemiology of Venezuelan hemorrhagic fever revealed extensive arenavirus infection (presumed to be caused by Guanarito virus) in two rodent species, Sigmodon alstoni and Zygodontomys brevicauda, collected from the region of Venezuela in which the disease is endemic. In the present study, four arenavirus isolates recovered from the Municipality of Guanarito (two isolates each from S. alstoni and Z. brevicauda) were characterized to learn more about the natural rodent host relationships of Guanarito virus. Serologic tests and analyses of nucleocapsid protein gene sequence data indicated that the two isolates from Z. brevicauda are strains of Guanarito virus and that the two isolates from S. alstoni are representatives of a novel New World arenavirus (proposed name Pirital) that is antigenically and phylogenetically distinct from all known New World arenaviruses. The results of the present study provide further evidence that the cane mouse Z. brevicauda is a natural host of Guanarito virus and suggest that the cotton rat S. alstoni is the natural reservoir host of Pirital but not Guanarito virus.

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