Detection of Circulating Antigen by Monoclonal Antibodies for Immunodiagnosis of Angiostrongyliasis

Soi-Moi Chye Department of Parasitology, Kaohsiung Medical College, Kaohsiung, Taiwan, Republic of China

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Chuan-Min Yen Department of Parasitology, Kaohsiung Medical College, Kaohsiung, Taiwan, Republic of China

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Eng-Rin Chen Department of Parasitology, Kaohsiung Medical College, Kaohsiung, Taiwan, Republic of China

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DOI:
https://doi.org/10.4269/ajtmh.1997.56.408
Volume/Issue:
Volume 56: Issue 4
Page(s):
408–412
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Two monoclonal antibodies, which recognize the epitope on an antigen with a molecular weight of 204 kD from the fifth-stage worm of Angiostrongylus cantonensis, were previously prepared and used to detect circulating antigens in patients with eosinophilic meningitis or meningoencephalitis and in mice experimentally infected with this parasite by a double-antibody, sandwich enzyme-linked immunosorbent assay (ELISA). The levels of this circulating antigen in experimentally infected mice were significantly higher three weeks after infection. The ELISA values in the detection of circulating antigens in cerebrospinal fluid (CSF) from patients were markedly higher than those in serum. Immunodiagnosis of patients with angiostrongyliasis by this technique proved to be highly specific for circulating antigens in serum and CSF specimens; however, the sensitivity in CSF was significantly higher than in serum.

Author Notes

Copyright:
Copyright © 1997 by The American Society of Tropical Medicine and Hygiene 1997
Published Online:
Apr 1997
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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