Leishmaniasis Research Group, Institute of Endemic Diseases, University of Khartoum, National Public Health Laboratory, Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, Centre for Medical Parasitology, University of Copenhagen, Khartoum, Sudan
A 45-year-old Sudanese man from western Sudan presented with oronasal leishmaniasis of three years duration. He had no history of previous kala-azar or cutaneous leishmaniasis. The parasite isolated from the oral mucosa was characterized by isoenzymes using 12 enzymes and by polymerase chain reaction amplification of kinetoplast DNA using species-specific primers. The specific primers gave products indistinguishable from those of the Leishmania donovani complex. However, the isoenzyme profile showed a zymodeme pattern that was significantly different from the zymodemes peviously reported in the Sudan and the Ethiopian region.