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A Quantitative Assay to Detect Circulating Fibrosin and Its Application in Experimental Schistosomiasis
David J. Wyler
David J. Wyler
Division of Geographic Medicine and Infectious Diseases, Tupper Research Institute, New England Medical Center, Boston, Massachusetts
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Pejman Talebian
Pejman Talebian
Division of Geographic Medicine and Infectious Diseases, Tupper Research Institute, New England Medical Center, Boston, Massachusetts
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The molecular pathogenesis of schistosomal liver fibrosis has been studied in a murine model of Schistosoma mansoni. A novel fibroblast growth factor, fibrosin, was identified as a product of a subpopulation of CD4+ lymphocytes resident in the hepatic egg granulomas. We describe a sensitive, quantitative, antigen-capture enzymelinked immunosorbent assay that can detect fibrosin in mouse serum and plasma at concentrations > 0.05 pg/ml. Using this assay, we detected in infected mice circulating fibrosin levels that were several fold increased above those detected in uninfected controls. Circulating fibrosin levels increased after week 4 of infection, reached a peak at week 8, and normalized by week 12. We propose that circulating fibrosin might be a useful marker of hepatic fibrogenesis in schistosomiasis.
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| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 920 | 682 | 31 |
| Full Text Views | 15 | 1 | 0 |
| PDF Downloads | 11 | 1 | 0 |