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Polymerase Chain Reaction for Diagnosis and Identification of Distinct Variants of Crimean-Congo Hemorrhagic Fever Virus in the United Arab Emirates
Tino F. Schwarz
Tino F. Schwarz
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Munich, Germany
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Herbert Nsanze
Herbert Nsanze
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Munich, Germany
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Maurice Longson
Maurice Longson
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Munich, Germany
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Hans Nitschko
Hans Nitschko
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Munich, Germany
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Sabine Gilch
Sabine Gilch
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Munich, Germany
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Hassan Shurie
Hassan Shurie
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Munich, Germany
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Abdulmajeed Ameen
Abdulmajeed Ameen
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Munich, Germany
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Abdul R. M. Zahir
Abdul R. M. Zahir
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Munich, Germany
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Upendra G. Acharya
Upendra G. Acharya
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Munich, Germany
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Gundula Jager
Gundula Jager
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Munich, Germany
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Viral hemorrhagic fever has re-emerged in the United Arab Emirates (UAE) since November 1993. Genomic RNA of Crimean-Congo hemorrhagic virus (C-CHFV) was detected by a newly developed, nested reverse transcriptase polymerase chain reaction (RT-PCR) in the sera of four (25.0%) of 16 suspected cases of viral hemorrhagic fever. The RT-PCR was based on oligonucleotide primers deducted from the small RNA segment encoding the nucleoprotein of the virus. By comparison with a nucleotide sequence of a C-CHFV isolate from a Chinese sheep, a divergence of 10.0–11.8% was detected in the C-CHFV variants causing the UAE outbreak. In the four positive sera, three phylogenetically distinct C-CHFV variants were amplified and confirmed by direct sequencing of the PCR fragments. These C-CHFV sequences were obtained directly from sera of infected humans without prior propagation in cell culture. The RT-PCR allows rapid detection of genomic C-CHFV RNA in clinical specimens and study of the molecular epidemiology of this infection.
| Past two years | Past Year | Past 30 Days | |
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