DNA Probes for the Anopheles punctulatus Complex

Nigel W. BeebeTropical Health Program, Queensland Institute of Medical Research, Tropical Health Program and the Department of Entomology, University of Queensland, Army Malaria Research Unit, Liverpool Military Area, Brisbane, Australia

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Desmond H. FoleyTropical Health Program, Queensland Institute of Medical Research, Tropical Health Program and the Department of Entomology, University of Queensland, Army Malaria Research Unit, Liverpool Military Area, Brisbane, Australia

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Robert D. CooperTropical Health Program, Queensland Institute of Medical Research, Tropical Health Program and the Department of Entomology, University of Queensland, Army Malaria Research Unit, Liverpool Military Area, Brisbane, Australia

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Joan H. BryanTropical Health Program, Queensland Institute of Medical Research, Tropical Health Program and the Department of Entomology, University of Queensland, Army Malaria Research Unit, Liverpool Military Area, Brisbane, Australia

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Allan SaulTropical Health Program, Queensland Institute of Medical Research, Tropical Health Program and the Department of Entomology, University of Queensland, Army Malaria Research Unit, Liverpool Military Area, Brisbane, Australia

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Genomic DNA probes were made for two recently identified members of the Anopheles punctulatus complex; Anopheles sp. near punctulatus from Papua New Guinea and Anopheles farauti No. 7 from the Solomon Islands. The probes are species-specific and with the use of 32P labeling sensitive enough so that a squash blot of only a small segment of the mosquito is required for identification. The 119-basepair (bp) probe for An. sp. near punctulatus and the 1,106-bp probe for An. farauti No. 7 have been sequenced in full and the probes have been tested on field collected specimens. These probes now make it possible to distinguish An. sp. near punctulatus and An. farauti No. 7 from the other eight members of the An. punctulatus complex. A pan-species probe was also made from the 18S ribosomal DNA that binds to DNA from all members of the complex. These three probes complete the set required for distinguishing all known members of the An. punctulatus complex by DNA hybridization.

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