Antigenic Components of Excretory-Secretory Products of Adult Fasciola hepatica Recognized in Human Infections

M. L. Sampaio SilvaLaboratorio de Parasitologia, Instituto Nacional de Saude, Dr. Ricardo Jorge, Laboratoire de Parasitologie Experimentale, Faculte de Medecine Pitie-Salpetriere, Porto, Portugal

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J. M. Correia da CostaLaboratorio de Parasitologia, Instituto Nacional de Saude, Dr. Ricardo Jorge, Laboratoire de Parasitologie Experimentale, Faculte de Medecine Pitie-Salpetriere, Porto, Portugal

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A. M. Viana da CostaLaboratorio de Parasitologia, Instituto Nacional de Saude, Dr. Ricardo Jorge, Laboratoire de Parasitologie Experimentale, Faculte de Medecine Pitie-Salpetriere, Porto, Portugal

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M. A. PiresLaboratorio de Parasitologia, Instituto Nacional de Saude, Dr. Ricardo Jorge, Laboratoire de Parasitologie Experimentale, Faculte de Medecine Pitie-Salpetriere, Porto, Portugal

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S. A. LopesLaboratorio de Parasitologia, Instituto Nacional de Saude, Dr. Ricardo Jorge, Laboratoire de Parasitologie Experimentale, Faculte de Medecine Pitie-Salpetriere, Porto, Portugal

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A. M. CastroLaboratorio de Parasitologia, Instituto Nacional de Saude, Dr. Ricardo Jorge, Laboratoire de Parasitologie Experimentale, Faculte de Medecine Pitie-Salpetriere, Porto, Portugal

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L. MonjourLaboratorio de Parasitologia, Instituto Nacional de Saude, Dr. Ricardo Jorge, Laboratoire de Parasitologie Experimentale, Faculte de Medecine Pitie-Salpetriere, Porto, Portugal

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The antigenic components of excretory-secretory products (ESP) of adult worms of Fasciola hepatica were revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis using sera from 20 patients infected with F. hepatica. Sera from 184 other parasitic infections and 20 healthy volunteers were also analyzed. It was found that the ESP were composed of more than 11 polypeptides; five components detected in fascioliasis sera had molecular weights of 12.4, 16.4, 19.4, 25, and 27 kilodaltons (kD). Only the 25- and 27-kD components were recognized by all 20 fascioliasis sera. Using the ESP as antigen, it was possible to perform an enzyme-linked immunosorbent assay with a sensitivity of 95% and a specificity of 97%. Sera from other parasitic infections had antibodies to antigenic components with apparent molecular weights of 37, 38.4, 52, 57, 63, 73, 87, 109, and 116 kD that were also found in sera from fascioliasis patients. These findings suggested that the 25- and 27-kD antigenic components may be sensitive and specific for the diagnosis of human fascioliasis.

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