Characterization of Human T Lymphocyte-Mediated Immune Responses Induced by a Vaccine against American Tegumentary Leishmaniasis

Sergio C. F. Mendonca Departamento de Protozoologia, Instituto Oswaldo Cruz, Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Rio de Janeiro, Brazil

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Paula M. De Luca Departamento de Protozoologia, Instituto Oswaldo Cruz, Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Rio de Janeiro, Brazil

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Wilson Mayrink Departamento de Protozoologia, Instituto Oswaldo Cruz, Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Rio de Janeiro, Brazil

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Teresa G. Restom Departamento de Protozoologia, Instituto Oswaldo Cruz, Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Rio de Janeiro, Brazil

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Fatima Conceicao-Silva Departamento de Protozoologia, Instituto Oswaldo Cruz, Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Rio de Janeiro, Brazil

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Alda Maria Da-Cruz Departamento de Protozoologia, Instituto Oswaldo Cruz, Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Rio de Janeiro, Brazil

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Alvaro L. Bertho Departamento de Protozoologia, Instituto Oswaldo Cruz, Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Rio de Janeiro, Brazil

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Carlos Alberto Da Costa Departamento de Protozoologia, Instituto Oswaldo Cruz, Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Rio de Janeiro, Brazil

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Odair Genaro Departamento de Protozoologia, Instituto Oswaldo Cruz, Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Rio de Janeiro, Brazil

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Vicente P. C. P. Toledo Departamento de Protozoologia, Instituto Oswaldo Cruz, Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Rio de Janeiro, Brazil

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Sergio G. Coutinho Departamento de Protozoologia, Instituto Oswaldo Cruz, Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Rio de Janeiro, Brazil

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DOI:
https://doi.org/10.4269/ajtmh.1995.53.195
Volume/Issue:
Volume 53: Issue 2
Page(s):
195ā€“201
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Forty-three Brazilians were immunized against American tegumentary leishmaniasis using a vaccine made of whole antigens from killed promastigotes of five American dermotropic Leishmania strains. None of the immunized subjects had a positive reaction in the Montenegro skin test (leishmanin) before vaccination, and 74% developed positive reactions in the skin test after vaccination. The proliferative responses of peripheral blood mononuclear cells (PBMC) induced by antigens from dermotropic Leishmania species were significantly higher after vaccination than before vaccination. However, with antigens from L. chagasi (a causative agent of American visceral leishmaniasis), there was no significant difference between the proliferative responses obtained before and after vaccination. Interferon-Ī³ was detected in the supernatants of L. braziliensis antigen-stimulated PBMC cultures after vaccination (but not before vaccination). One year after vaccination, PBMC were obtained from eight of the immunized individuals and stimulated with L. braziliensis antigens in proliferative response assays. In all cases, the majority of the responding cells were CD8+ T cells, in contrast to the results of a group of patients with active lesions of tegumentary leishmaniasis, whose L. braziliensis-reactive cells were mainly of the CD4+ T cell phenotype.
Copyright:
Copyright Ā© 1995 by The American Society of Tropical Medicine and Hygiene 1995
Published Online:
Aug 1995
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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