Protective Immunity Induced in Squirrel Monkeys with Recombinant Apical Membrane Antigen-1 of Plasmodium fragile

William E. Collins Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Prevention & Control, CSL Ltd, The Walter and Eliza Hall Institute of Medical Research, Atlanta, Georgia

Search for other papers by William E. Collins in
Current site
Google Scholar
PubMed Close
,
David Pye Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Prevention & Control, CSL Ltd, The Walter and Eliza Hall Institute of Medical Research, Atlanta, Georgia

Search for other papers by David Pye in
Current site
Google Scholar
PubMed Close
,
Pauline E. Crewther Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Prevention & Control, CSL Ltd, The Walter and Eliza Hall Institute of Medical Research, Atlanta, Georgia

Search for other papers by Pauline E. Crewther in
Current site
Google Scholar
PubMed Close
,
Kirsten L. Vandenberg Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Prevention & Control, CSL Ltd, The Walter and Eliza Hall Institute of Medical Research, Atlanta, Georgia

Search for other papers by Kirsten L. Vandenberg in
Current site
Google Scholar
PubMed Close
,
G. Gale Galland Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Prevention & Control, CSL Ltd, The Walter and Eliza Hall Institute of Medical Research, Atlanta, Georgia

Search for other papers by G. Gale Galland in
Current site
Google Scholar
PubMed Close
,
Alexander J. Sulzer Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Prevention & Control, CSL Ltd, The Walter and Eliza Hall Institute of Medical Research, Atlanta, Georgia

Search for other papers by Alexander J. Sulzer in
Current site
Google Scholar
PubMed Close
,
David J. Kemp Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Prevention & Control, CSL Ltd, The Walter and Eliza Hall Institute of Medical Research, Atlanta, Georgia

Search for other papers by David J. Kemp in
Current site
Google Scholar
PubMed Close
,
Stirling J. Edwards Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Prevention & Control, CSL Ltd, The Walter and Eliza Hall Institute of Medical Research, Atlanta, Georgia

Search for other papers by Stirling J. Edwards in
Current site
Google Scholar
PubMed Close
,
Ross L. Coppel Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Prevention & Control, CSL Ltd, The Walter and Eliza Hall Institute of Medical Research, Atlanta, Georgia

Search for other papers by Ross L. Coppel in
Current site
Google Scholar
PubMed Close
,
Joann S. Sullivan Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Prevention & Control, CSL Ltd, The Walter and Eliza Hall Institute of Medical Research, Atlanta, Georgia

Search for other papers by Joann S. Sullivan in
Current site
Google Scholar
PubMed Close
,
Carla L. Morris Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Prevention & Control, CSL Ltd, The Walter and Eliza Hall Institute of Medical Research, Atlanta, Georgia

Search for other papers by Carla L. Morris in
Current site
Google Scholar
PubMed Close
, and
Robin F. Anders Division of Parasitic Diseases and Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Prevention & Control, CSL Ltd, The Walter and Eliza Hall Institute of Medical Research, Atlanta, Georgia

Search for other papers by Robin F. Anders in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.4269/ajtmh.1994.51.711
Volume/Issue:
Volume 51: Issue 6
Page(s):
711–719
Restricted access
Get Permissions

Saimiri sciureus boliviensis monkeys were immunized with the Plasmodium fragile form of the merozoite apical membrane antigen-1 produced using the baculovirus expression system and combined with Montanide ISA 720 adjuvant. Following three immunizations, monkeys were challenged with 10,000 P. fragile trophozoite parasites. Antibody titers determined by fluorescence microscopy indicated an enhanced response following the second immunization. Four of five control animals had parasite counts > 5% 18–26 days following challenge. Four of five immunized monkeys had reduced levels of maximum parasitemia or delays in accumulated parasite counts, suggestive of protection. Rechallenge of the animals with P. falciparum resulted in three of four adjuvant control animals developing patent parasitemia whereas none of five immunized animals were infected, suggesting some level of heterologous protection.

Author Notes

Copyright:
Copyright © 1994 by The American Society of Tropical Medicine and Hygiene 1994
Published Online:
Dec 1994
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Past two years Past Year Past 30 Days
Abstract Views 1919 1458 445
Full Text Views 29 6 0
PDF Downloads 25 6 0
 

 

 

 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save