Allelic Variation in the Circumsporozoite Protein of Plasmodium falciparum from Thai Field Isolates

Somchai Jongwutiwes Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Laboratory of Biology, Osaka Institute of Technology, Department of Biology, Institute of Molecular Evolutionary Genetics, The Pennsylvania State University, Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, Bangkok, Thailand

Search for other papers by Somchai Jongwutiwes in
Current site
Google Scholar
PubMed Close
,
Kazuyuki Tanabe Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Laboratory of Biology, Osaka Institute of Technology, Department of Biology, Institute of Molecular Evolutionary Genetics, The Pennsylvania State University, Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, Bangkok, Thailand

Search for other papers by Kazuyuki Tanabe in
Current site
Google Scholar
PubMed Close
,
Marianne K. Hughes Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Laboratory of Biology, Osaka Institute of Technology, Department of Biology, Institute of Molecular Evolutionary Genetics, The Pennsylvania State University, Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, Bangkok, Thailand

Search for other papers by Marianne K. Hughes in
Current site
Google Scholar
PubMed Close
,
Hiroji Kanbara Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Laboratory of Biology, Osaka Institute of Technology, Department of Biology, Institute of Molecular Evolutionary Genetics, The Pennsylvania State University, Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, Bangkok, Thailand

Search for other papers by Hiroji Kanbara in
Current site
Google Scholar
PubMed Close
, and
Austin L. Hughes Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Laboratory of Biology, Osaka Institute of Technology, Department of Biology, Institute of Molecular Evolutionary Genetics, The Pennsylvania State University, Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, Bangkok, Thailand

Search for other papers by Austin L. Hughes in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.4269/ajtmh.1994.51.659
Volume/Issue:
Volume 51: Issue 5
Page(s):
659–668
Restricted access
Get Permissions

Allelic variation in the Plasmodium falciparum circumsporozoite (CS) protein gene has been examined by sequencing the entire gene in 15 isolates from an endemic area of Thailand. The isolates contain a total of six new allelic forms of the tetrapeptide repeats and eight variants of the T cell epitope (TCE) region of the CS gene. All nucleotide substitutions in the TCE are nonsynonymous. There is no apparent association between the sequence patterns in the repeats and in the TCE. Comparison of the TCE with published sequences has shown that most variants of our isolates are not identical to those found in different geographic areas, suggesting geographic variation in genetic diversity of the CS protein. In a phylogenetic tree, the new Thai alleles did not cluster together, suggesting a considerable heterogeneity within some geographic areas. Furthermore, analyses of tetrapeptide repeats from a number of isolates and strains showed evidence of three genetic mechanisms for the generation of variation in the repeats of the CS gene: point mutation, duplication of one or more repeat units, and intragenic recombination.

Author Notes

Copyright:
Copyright © 1994 by The American Society of Tropical Medicine and Hygiene 1994
Published Online:
Nov 1994
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Past two years Past Year Past 30 Days
Abstract Views 568 375 23
Full Text Views 16 1 0
PDF Downloads 16 2 0
 
 
 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save