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Isolation of a Strain of a Hantaan Virus from a Fatal Case of Hemorrhagic Fever with Renal Syndrome in Slovenia
Tatjana Avsic-Zupanc
Tatjana Avsic-Zupanc
Institute of Microbiology, Medical Faculty of Ljubljana, Regional Hospital Novo Mesto, National Institute of Allergy and Infectious Diseases, National Institutes of Health, United States Army Medical Research Institute of Infectious Diseases, Ljubljana, Slovenia
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Mario Poljak
Mario Poljak
Institute of Microbiology, Medical Faculty of Ljubljana, Regional Hospital Novo Mesto, National Institute of Allergy and Infectious Diseases, National Institutes of Health, United States Army Medical Research Institute of Infectious Diseases, Ljubljana, Slovenia
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Polona Furlan
Polona Furlan
Institute of Microbiology, Medical Faculty of Ljubljana, Regional Hospital Novo Mesto, National Institute of Allergy and Infectious Diseases, National Institutes of Health, United States Army Medical Research Institute of Infectious Diseases, Ljubljana, Slovenia
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Rafael Kaps
Rafael Kaps
Institute of Microbiology, Medical Faculty of Ljubljana, Regional Hospital Novo Mesto, National Institute of Allergy and Infectious Diseases, National Institutes of Health, United States Army Medical Research Institute of Infectious Diseases, Ljubljana, Slovenia
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Shu-Yuan Xiao
Shu-Yuan Xiao
Institute of Microbiology, Medical Faculty of Ljubljana, Regional Hospital Novo Mesto, National Institute of Allergy and Infectious Diseases, National Institutes of Health, United States Army Medical Research Institute of Infectious Diseases, Ljubljana, Slovenia
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James W. Leduc
James W. Leduc
Institute of Microbiology, Medical Faculty of Ljubljana, Regional Hospital Novo Mesto, National Institute of Allergy and Infectious Diseases, National Institutes of Health, United States Army Medical Research Institute of Infectious Diseases, Ljubljana, Slovenia
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Hemorrhagic fever with renal syndrome (HFRS) has been serologically confirmed in Slovenia during the last seven years. There is evidence that three hantaviruses (Hantaan, Puumala, and a newly described form termed Dobrava) circulate simultaneously in this area. Recently, a hantavirus was isolated from the urine and brain tissue of a fatal case of HFRS. Positive immunofluorescent reactions with reference human sera and monoclonal antibodies were first recognized after the second cell culture passage. Extensive cross-reactivity between our isolate and prototype Hantaan virus, strain 76–118, and Hantaan-like isolates from the former Yugoslavia, Fojnica and Plitvice, was revealed by enzyme-linked immunosorbent assay with specific rat antisera. The reaction pattern of the isolate was similar to the prototype Hantaan virus by indirect immunofluorescent assay with a panel of monoclonal antibodies. Furthermore, the specificity of the isolates was confirmed by analysis of polymerase chain reaction products of this virus with five restriction endonucleases. This appears to be the first isolation of a strain of prototype Hantaan virus from a fatal case of HFRS in Europe.
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