Determination of Fifty Percent Inhibitory Concentrations (IC50) of Antimalarial Drugs against Plasmodium Falciparum Parasites in a Serum-Free Medium

Ayub O. Ofulla Biomedical Sciences Research Center and Clinical Research Center, Kenya Medical Research Institute, Zoology Department, Kenyatta University, Infectious Diseases Division, Naval Medical Research Institute, USAMRU-Kenya, Walter Reed Army Institute of Research, Nairobi, Kenya

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Aloys S. Orago Biomedical Sciences Research Center and Clinical Research Center, Kenya Medical Research Institute, Zoology Department, Kenyatta University, Infectious Diseases Division, Naval Medical Research Institute, USAMRU-Kenya, Walter Reed Army Institute of Research, Nairobi, Kenya

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John I. Githure Biomedical Sciences Research Center and Clinical Research Center, Kenya Medical Research Institute, Zoology Department, Kenyatta University, Infectious Diseases Division, Naval Medical Research Institute, USAMRU-Kenya, Walter Reed Army Institute of Research, Nairobi, Kenya

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James P. Burans Biomedical Sciences Research Center and Clinical Research Center, Kenya Medical Research Institute, Zoology Department, Kenyatta University, Infectious Diseases Division, Naval Medical Research Institute, USAMRU-Kenya, Walter Reed Army Institute of Research, Nairobi, Kenya

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Gladys M. Aleman Biomedical Sciences Research Center and Clinical Research Center, Kenya Medical Research Institute, Zoology Department, Kenyatta University, Infectious Diseases Division, Naval Medical Research Institute, USAMRU-Kenya, Walter Reed Army Institute of Research, Nairobi, Kenya

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Anthony J. Johnson Biomedical Sciences Research Center and Clinical Research Center, Kenya Medical Research Institute, Zoology Department, Kenyatta University, Infectious Diseases Division, Naval Medical Research Institute, USAMRU-Kenya, Walter Reed Army Institute of Research, Nairobi, Kenya

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Samuel K. Martin Biomedical Sciences Research Center and Clinical Research Center, Kenya Medical Research Institute, Zoology Department, Kenyatta University, Infectious Diseases Division, Naval Medical Research Institute, USAMRU-Kenya, Walter Reed Army Institute of Research, Nairobi, Kenya

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DOI:
https://doi.org/10.4269/ajtmh.1994.51.214
Volume/Issue:
Volume 51: Issue 2
Page(s):
214–218
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Plasmodium falciparum chemosensitivity to the various antimalarial drugs is presently determined in the laboratory by setting up multiple microcultures of the parasite and estimating the amount of growth inhibition caused by known concentrations of drug. Parasite growth inhibition is assessed either by microscopy, radiolabeled substrate uptake, or calorimetrically. The obligate requirement for serum in this assay presents difficulties in the direct comparison of results among laboratories. We now have evidence that antimalarial drug sensitivity assays can be reliably performed in a serum-free medium. The overall comparison of 50% inhibitory concentration (IC50) values obtained with serum-free media (bovine albumin, Cohn fraction V [BAM] and BAM combined with glucose and lipids-cholesterol-rich mixture) and those obtained in serum-supplemented medium was r = 0.56; n = 60; P < 0.01.

Author Notes

Copyright:
Copyright © 1994 by The American Society of Tropical Medicine and Hygiene 1994
Published Online:
Aug 1994
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637

Expert Committee on Hepatitis

First Report. World Health Organization Technical Report Series No. 62 (Available also in French). 26 pp. Geneva, W.H.O., Palais des Nations. March, 1953. Price 1/6, $0.20, Fr. fr. 65.-; Sw. fr. 0.80

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