Antigenic Characterization of Plasmodium Vivax with Monoclonal Antibodies

Maria Reyna Sanchez Departmento de Inmunologia, Instituto de Investigaciones Biomedicas, University Nacional Autonoma de Mexico, Instituto Nacional de la Nutricion Salvador Zubiran, Centro de Investigacion de Paludismo, Tapachula, Chiapas, Mexico

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Jose Antonio Ramirez Departmento de Inmunologia, Instituto de Investigaciones Biomedicas, University Nacional Autonoma de Mexico, Instituto Nacional de la Nutricion Salvador Zubiran, Centro de Investigacion de Paludismo, Tapachula, Chiapas, Mexico

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Jorge Larriva-Sahd Departmento de Inmunologia, Instituto de Investigaciones Biomedicas, University Nacional Autonoma de Mexico, Instituto Nacional de la Nutricion Salvador Zubiran, Centro de Investigacion de Paludismo, Tapachula, Chiapas, Mexico

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Mario H. Rodriguez Departmento de Inmunologia, Instituto de Investigaciones Biomedicas, University Nacional Autonoma de Mexico, Instituto Nacional de la Nutricion Salvador Zubiran, Centro de Investigacion de Paludismo, Tapachula, Chiapas, Mexico

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Raul Mancilla Departmento de Inmunologia, Instituto de Investigaciones Biomedicas, University Nacional Autonoma de Mexico, Instituto Nacional de la Nutricion Salvador Zubiran, Centro de Investigacion de Paludismo, Tapachula, Chiapas, Mexico

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Librado Ortiz-Ortiz Departmento de Inmunologia, Instituto de Investigaciones Biomedicas, University Nacional Autonoma de Mexico, Instituto Nacional de la Nutricion Salvador Zubiran, Centro de Investigacion de Paludismo, Tapachula, Chiapas, Mexico

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Monoclonal antibodies were produced against Plasmodium vivax obtained from patients living in southeastern Mexico, where P. vivax malaria is endemic. Nine hybridomas specific for this parasite were obtained. By an indirect immunofluorescence assay, seven antibodies were found to react with epitopes present in the cytoplasm of the infected erythrocyte and two with the parasite itself. By immunoblotting, five monoclonal antibodies reacted with a 17-kD protein band, three with an 85-kD band, and two with one of 45 kD. By immunogold electron microscopy, two antibodies that reacted with the cytoplasm of infected erythrocytes by immunofluorescence also labeled cytoplasmic clefts, and one, in addition, recognized caveola-vesicle complexes and the parasite matrix. These results demonstrate the value of monoclonal antibodies in identifying P. vivax antigens and disclosing their subcellular distribution.

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