Detection of the HI-j Strain of Salmonella typhi among Korean Isolates by the Polymerase Chain Reaction

Jae-Hoon Song Departments of Internal Medicine, Microbiology, and Clinical Pathology, Asan Medical Center, College of Medicine, University of Ulsan, Asan Institute for Life Sciences, Seoul, Republic of Korea

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Helen Cho Departments of Internal Medicine, Microbiology, and Clinical Pathology, Asan Medical Center, College of Medicine, University of Ulsan, Asan Institute for Life Sciences, Seoul, Republic of Korea

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Mee Yeon Park Departments of Internal Medicine, Microbiology, and Clinical Pathology, Asan Medical Center, College of Medicine, University of Ulsan, Asan Institute for Life Sciences, Seoul, Republic of Korea

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Yang Soo Kim Departments of Internal Medicine, Microbiology, and Clinical Pathology, Asan Medical Center, College of Medicine, University of Ulsan, Asan Institute for Life Sciences, Seoul, Republic of Korea

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Hee Bom Moon Departments of Internal Medicine, Microbiology, and Clinical Pathology, Asan Medical Center, College of Medicine, University of Ulsan, Asan Institute for Life Sciences, Seoul, Republic of Korea

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Yoo Kyum Kim Departments of Internal Medicine, Microbiology, and Clinical Pathology, Asan Medical Center, College of Medicine, University of Ulsan, Asan Institute for Life Sciences, Seoul, Republic of Korea

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Chik Hyun Pai Departments of Internal Medicine, Microbiology, and Clinical Pathology, Asan Medical Center, College of Medicine, University of Ulsan, Asan Institute for Life Sciences, Seoul, Republic of Korea

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DOI:
https://doi.org/10.4269/ajtmh.1994.50.608
Volume/Issue:
Volume 50: Issue 5
Page(s):
608–611
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Salmonella typhi, the etiologic agent of typhoid fever, typically has only a phase-1 flagellar antigen, HI-d (fliC). While most strains of S. typhi have HI-d antigen, 10–20% of Indonesian isolates have been reported to possess HI-j antigen instead. To investigate the presence HI-j strains of S. typhi isolates in Korea, where typhoid fever is still a common infectious problem, we used the polymerase chain reaction (PCR) with a pair of oligonucleotides primers that specifically amplified the flagellin gene of S. typhi. Of 375 isolates of S. typhi tested, only one was shown to possess the HI-j antigen, which was shown by the presence of a 1,269-basepair fragment on agarose gel electrophoresis after the PCR. The isolate with the HI-j antigen was cultured from a Korean-Indonesian man who was already symptomatic in Indonesia and was thought to be an Indonesian strain. Because 375 strains tested in this study were collected from cases with typhoid fever in different regions of Korea during the period from 1986 to 1991, it could be concluded that the mutation rate to j antigen is negligible among S. typhi endemic in Korea.

Author Notes

Copyright:
Copyright © 1994 by The American Society of Tropical Medicine and Hygiene 1994
Published Online:
May 1994
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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