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DNA Probes for Identifying the Members of the Anopheles punctulatus Complex in Papua New Guinea
Nigel W. Beebe
Nigel W. Beebe
Tropical Health Program, Queensland Institute of Medical Research, Brisbane, Tropical Health Program and Entomology Department, University of Queensland, Queensland, Australia
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Desmond H. Foley
Desmond H. Foley
Tropical Health Program, Queensland Institute of Medical Research, Brisbane, Tropical Health Program and Entomology Department, University of Queensland, Queensland, Australia
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Allan Saul
Allan Saul
Tropical Health Program, Queensland Institute of Medical Research, Brisbane, Tropical Health Program and Entomology Department, University of Queensland, Queensland, Australia
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Leanne Cooper
Leanne Cooper
Tropical Health Program, Queensland Institute of Medical Research, Brisbane, Tropical Health Program and Entomology Department, University of Queensland, Queensland, Australia
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Joan H. Bryan
Joan H. Bryan
Tropical Health Program, Queensland Institute of Medical Research, Brisbane, Tropical Health Program and Entomology Department, University of Queensland, Queensland, Australia
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Thomas R. Burkot
Thomas R. Burkot
Tropical Health Program, Queensland Institute of Medical Research, Brisbane, Tropical Health Program and Entomology Department, University of Queensland, Queensland, Australia
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Genomic DNA probes were made for five members of the Anopheles punctulatus complex of mosquitoes found in Papua New Guinea. Specific DNA probes were developed for An. punctulatus, An. koliensis, and three sibling species, An. farauti No. 4, An. farauti No. 5, and An. farauti No. 6, by differentially screening total genomic DNA libraries of individual species and sibling species with homologous DNA against heterologous DNA labeled with 32P. Probes ranged from 273 to 630 bp. Identification of species can be made from squash or dot blots using only a segment of the mosquito (i.e., head, thorax, abdomen, or even legs), allowing for concurrent analysis of the remainder of the mosquito for other epidemiologic characteristics.
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| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 605 | 382 | 12 |
| Full Text Views | 18 | 3 | 1 |
| PDF Downloads | 20 | 7 | 2 |