By Everard L. Napier, M.R.C.S., L.R.C.P. (Lond.). In charge Kala-azar research, Calcutta School of Tropical Medicine. Second edition. 185 pages of text with 15 charts in the text, 18 plates, and an appendix of references to literature, author index and subject index. Oxford University Press. London, Bombay, Calcutta, Madras, 1927
Entamoeba histolytica was cultivated with a mixed bacterial flora through 15 transfers in 30 days in a medium containing inorganic salts, rice powder, glucosamine hydrochloride, and gelatin. The glucosamine could not be replaced by glucose, nor by any of a series of organic amines and amino alcohols. Gelatin could be replaced by serum proteins and the enzymatic digests of certain proteins. It could not be replaced by casein, edestin, lactalbumin, blood fibrin, nor by glycine or a mixture of amino acids based on the composition of gelatin.