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Longitudinal Study of Naturally Acquired Humoral Immune Responses against the Merozoite Surface Protein 1 of Plasmodium vivax in Patients from Rondonia, Brazil
Frederic Mertens
Frederic Mertens
Departamento de Parasitologia (ICB II), Universidade de Sao Paulo, Laboratorio de Malaria, Superintendencia de Controle de Endemias (SUCEM), Sao Paulo, Brazil
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Gabriela Levitus
Gabriela Levitus
Departamento de Parasitologia (ICB II), Universidade de Sao Paulo, Laboratorio de Malaria, Superintendencia de Controle de Endemias (SUCEM), Sao Paulo, Brazil
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Luiz-Marcelo Aranha Camargo
Luiz-Marcelo Aranha Camargo
Departamento de Parasitologia (ICB II), Universidade de Sao Paulo, Laboratorio de Malaria, Superintendencia de Controle de Endemias (SUCEM), Sao Paulo, Brazil
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Marcelo Urbano Ferreira
Marcelo Urbano Ferreira
Departamento de Parasitologia (ICB II), Universidade de Sao Paulo, Laboratorio de Malaria, Superintendencia de Controle de Endemias (SUCEM), Sao Paulo, Brazil
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Araripe Pacheco Dutra
Araripe Pacheco Dutra
Departamento de Parasitologia (ICB II), Universidade de Sao Paulo, Laboratorio de Malaria, Superintendencia de Controle de Endemias (SUCEM), Sao Paulo, Brazil
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Hernando A. Del Portillo
Hernando A. Del Portillo
Departamento de Parasitologia (ICB II), Universidade de Sao Paulo, Laboratorio de Malaria, Superintendencia de Controle de Endemias (SUCEM), Sao Paulo, Brazil
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A longitudinal study on the naturally acquired humoral immune responses against the merozoite surface protein 1 of Plasmodium vivax (PvMSP-1) was performed in malaria patients from the Brazilian Amazon region of Rondonia. We have previously cloned and expressed a recombinant protein, ICB2-5, that encodes 508 amino acids from the N-terminal portion of the PvMSP-1 protein. This affinity-purified polypeptide was tested by an enzyme-linked immunosorbent assay in a one-year longitudinal study using sera from 34 patients who had at least one malaria infection during the study period. The results demonstrated that more than 90% of the sera from patients having experienced more than three previous malaria infections contained antibodies to ICB2-5 at the time of a new clinical episode. Unexpectedly, more than half of these multiple-infected patients had an antibody response to ICB2-5 in which the predominant isotype was IgM. In contrast, more than 83% of the sera from these same patients contained predominantly IgG antibodies against total blood-stage antigen preparations. To determine if these results were due to the lack of boosting against this portion of the PvMSP-1 molecule, the presence of IgG antibodies to ICB2-5 in the sera from 11 patients who had consecutive malarial episodes during the study year was investigated. Five of these eleven patients failed to produce IgG antibodies to ICB2-5 even after 1–3 infections. Thus, these results suggest that no boosting against this region of the PvMSP-1 molecule was achieved by natural infections among these patients.
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| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 868 | 531 | 112 |
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