| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 556 | 351 | 175 |
| Full Text Views | 12 | 3 | 0 |
| PDF Downloads | 11 | 4 | 0 |
Humoral and Cellular Immune Responses to the Circumsporozoite Protein of Plasmodium vivax in Madagascar
Florence Migot
Florence Migot
INSERM U13/Institut de Medecine et d'Epidemiologie Africaines, Hopital Claude Bernard, Service de Parasitologie-Mycologie, Faculte de Medecine (Lille II) et de Pharmacie (Paris V), Malaria Branch, Centers for Disease Control, Institut Pasteur de Madagascar, Paris, France
Search for other papers by Florence Migot in
Current site
Google Scholar
PubMed
Search for other papers by Florence Migot in
Current site
Google Scholar
PubMed
Pascal Millet
Pascal Millet
INSERM U13/Institut de Medecine et d'Epidemiologie Africaines, Hopital Claude Bernard, Service de Parasitologie-Mycologie, Faculte de Medecine (Lille II) et de Pharmacie (Paris V), Malaria Branch, Centers for Disease Control, Institut Pasteur de Madagascar, Paris, France
Search for other papers by Pascal Millet in
Current site
Google Scholar
PubMed
Search for other papers by Pascal Millet in
Current site
Google Scholar
PubMed
Claire Chougnet
Claire Chougnet
INSERM U13/Institut de Medecine et d'Epidemiologie Africaines, Hopital Claude Bernard, Service de Parasitologie-Mycologie, Faculte de Medecine (Lille II) et de Pharmacie (Paris V), Malaria Branch, Centers for Disease Control, Institut Pasteur de Madagascar, Paris, France
Search for other papers by Claire Chougnet in
Current site
Google Scholar
PubMed
Search for other papers by Claire Chougnet in
Current site
Google Scholar
PubMed
Jean-Paul Lepers
Jean-Paul Lepers
INSERM U13/Institut de Medecine et d'Epidemiologie Africaines, Hopital Claude Bernard, Service de Parasitologie-Mycologie, Faculte de Medecine (Lille II) et de Pharmacie (Paris V), Malaria Branch, Centers for Disease Control, Institut Pasteur de Madagascar, Paris, France
Search for other papers by Jean-Paul Lepers in
Current site
Google Scholar
PubMed
Search for other papers by Jean-Paul Lepers in
Current site
Google Scholar
PubMed
Philippe Deloron
Philippe Deloron
INSERM U13/Institut de Medecine et d'Epidemiologie Africaines, Hopital Claude Bernard, Service de Parasitologie-Mycologie, Faculte de Medecine (Lille II) et de Pharmacie (Paris V), Malaria Branch, Centers for Disease Control, Institut Pasteur de Madagascar, Paris, France
Search for other papers by Philippe Deloron in
Current site
Google Scholar
PubMed
Search for other papers by Philippe Deloron in
Current site
Google Scholar
PubMed
Plasmodium vivax malaria is prevalent during the rainy season in the central highlands of Madagascar. In April 1991, we investigated the cellular and antibody immune responses of 53 inhabitants of Manarintsoa, a village in this area, to four antigens corresponding to B and T cell epitopes of the P. vivax circumsporozoite (CS) protein. Cellular responses were assessed by lymphocyte proliferation assay as well as by detection of interferon-gamma and interleukin-2 production in vitro. Cell culture was performed with two overlapping synthetic peptides (CSVTCGVGVRVRSRVNA [amino acids 311–326]) and VRVRSRVNAANKKPED [amino acids 319–334]) from the vicinity of the highly conserved region II of the CS protein. In at least one of the three assays, cells from seven subjects showed a positive response to CSVTCGVGVRVRSRVNA, while cells form 14 subjects responded to VRVRSRVNAANKKPED. Antibodies directed against the two recombinant antigens, NS181V20 and rPvCS-2, both of which contain the entire central repeat region of the P. vivax CS protein, plus regions I and II in the case of rPvCS-2, were measured by the Falcon® assay screening test-enzyme-linked immunosorbent assay. Eight and nine subjects had antibodies to NS181V20 and rPvCS-2, respectively. The presence of antibody responses to both recombinant antigens was related (P = 0.02, by Fisher's exact test), but was not related to the presence of a cellular response to peptides from vicinity of region II (P > 0.1, by Fisher's exact test). These data show that most of the antibody response is probably directed towards the repeat region of the CS protein of P. vivax, but that the vicinity of region II is able to induce both antibody and cellular responses in some individuals.
Author Notes
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 556 | 351 | 175 |
| Full Text Views | 12 | 3 | 0 |
| PDF Downloads | 11 | 4 | 0 |