An Improved Microassay for Plasmodium falciparum Cytoadherence using Stable Transformants of Chinese Hamster Ovary Cells Expressing CD36 or Intercellular Adhesion Molecule-1

Thomas HaslerLaboratory of Infectious Diseases, DNAX Research Institute, Department of Medical and Molecular Parasitology, New York University Medical Center, Medical Research Council Laboratories, Palo Alto, California, The Gambia

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Glenn R. AlbrechtLaboratory of Infectious Diseases, DNAX Research Institute, Department of Medical and Molecular Parasitology, New York University Medical Center, Medical Research Council Laboratories, Palo Alto, California, The Gambia

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Marie Rose Van SchravendijkLaboratory of Infectious Diseases, DNAX Research Institute, Department of Medical and Molecular Parasitology, New York University Medical Center, Medical Research Council Laboratories, Palo Alto, California, The Gambia

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Joao C. AguiarLaboratory of Infectious Diseases, DNAX Research Institute, Department of Medical and Molecular Parasitology, New York University Medical Center, Medical Research Council Laboratories, Palo Alto, California, The Gambia

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Kerstin E. MoreheadLaboratory of Infectious Diseases, DNAX Research Institute, Department of Medical and Molecular Parasitology, New York University Medical Center, Medical Research Council Laboratories, Palo Alto, California, The Gambia

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Brittan L. PasloskeLaboratory of Infectious Diseases, DNAX Research Institute, Department of Medical and Molecular Parasitology, New York University Medical Center, Medical Research Council Laboratories, Palo Alto, California, The Gambia

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Cynthia MaLaboratory of Infectious Diseases, DNAX Research Institute, Department of Medical and Molecular Parasitology, New York University Medical Center, Medical Research Council Laboratories, Palo Alto, California, The Gambia

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John W. BarnwellLaboratory of Infectious Diseases, DNAX Research Institute, Department of Medical and Molecular Parasitology, New York University Medical Center, Medical Research Council Laboratories, Palo Alto, California, The Gambia

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Brian GreenwoodLaboratory of Infectious Diseases, DNAX Research Institute, Department of Medical and Molecular Parasitology, New York University Medical Center, Medical Research Council Laboratories, Palo Alto, California, The Gambia

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Russell J. HowardLaboratory of Infectious Diseases, DNAX Research Institute, Department of Medical and Molecular Parasitology, New York University Medical Center, Medical Research Council Laboratories, Palo Alto, California, The Gambia

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Stable transformants of Chinese hamster ovary (CHO) cell lines expressing high levels of human CD36 or intercellular adhesion molecule-1 (ICAM-1) have been produced as target cells for cytoadherence of Plasmodium falciparum-infected erythrocytes. An improved adherence microassay has been designed using small sample volumes and allowing convenient and reliable measurements on a large number of samples. The assay can be used both with purified proteins spotted on plastic and with the stably transformed CHO cell lines. The same assay plate can be evaluated either microscopically or by scintillation counting after use of 3H-hypoxanthine-labeled parasites. Using the microassay, functional expression of the transfected receptor molecules on CHO-CD36 and CHO-ICAM was confirmed using parasites with different cytoadherence phenotypes and cytoadherence inhibition experiments with a panel of anti-CD36 antibodies. The use of isolates from The Gambia confirmed the applicability of these assays for laboratory studies of these isolates.

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