Author:
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 499 | 235 | 5 |
| Full Text Views | 17 | 0 | 0 |
| PDF Downloads | 17 | 0 | 0 |
Improved Diagnostic Performance of the Circulating Antigen Assay in Human Schistosomiasis by Parallel Testing for Circulating Anodic and Cathodic Antigens in Serum and Urine
Lisette Van Lieshout
Lisette Van Lieshout
U. S. Naval Medical Research Unit No. 3, Laboratory of Parasitology, University of Leiden, Cairo, Egypt
Search for other papers by Lisette Van Lieshout in
Current site
Google Scholar
PubMed
Search for other papers by Lisette Van Lieshout in
Current site
Google Scholar
PubMed
Niels De Jonge
Niels De Jonge
U. S. Naval Medical Research Unit No. 3, Laboratory of Parasitology, University of Leiden, Cairo, Egypt
Search for other papers by Niels De Jonge in
Current site
Google Scholar
PubMed
Search for other papers by Niels De Jonge in
Current site
Google Scholar
PubMed
Nabil A. El Masry
Nabil A. El Masry
U. S. Naval Medical Research Unit No. 3, Laboratory of Parasitology, University of Leiden, Cairo, Egypt
Search for other papers by Nabil A. El Masry in
Current site
Google Scholar
PubMed
Search for other papers by Nabil A. El Masry in
Current site
Google Scholar
PubMed
Moustafa M. Mansour
Moustafa M. Mansour
U. S. Naval Medical Research Unit No. 3, Laboratory of Parasitology, University of Leiden, Cairo, Egypt
Search for other papers by Moustafa M. Mansour in
Current site
Google Scholar
PubMed
Search for other papers by Moustafa M. Mansour in
Current site
Google Scholar
PubMed
Frederik W. Krijger
Frederik W. Krijger
U. S. Naval Medical Research Unit No. 3, Laboratory of Parasitology, University of Leiden, Cairo, Egypt
Search for other papers by Frederik W. Krijger in
Current site
Google Scholar
PubMed
Search for other papers by Frederik W. Krijger in
Current site
Google Scholar
PubMed
Andre M. Deelder
Andre M. Deelder
U. S. Naval Medical Research Unit No. 3, Laboratory of Parasitology, University of Leiden, Cairo, Egypt
Search for other papers by Andre M. Deelder in
Current site
Google Scholar
PubMed
Search for other papers by Andre M. Deelder in
Current site
Google Scholar
PubMed
Serum and urine levels of two Schistosoma circulating antigens, the circulating anodic antigen (CAA) and the circulating cathodic antigen (CCA), were determined by monoclonal antibody-based enzyme-linked immunosorbent assays in 56 Egyptian patients infected with S. mansoni and in 12 patients infected with both S. mansoni and S. haematobium. Both CAA and CCA could be specifically demonstrated in 82% and 88% of the serum samples and in 88% and 87% of the urine samples, respectively. While complete specificity was maintained, sensitivity was increased to a range of 91–98% by parallel use of the two circulating antigen assays, i.e., an individual with a positive titer for at least one of the assays was considered to be infected. A combination of CAA and CCA determinations in urine samples only resulted in a sensitivity of 94%. However, the highest sensitivity was achieved when the serum-CCA assay was combined with the urine-CCA assay (98%) or with the urine-CAA assay (97%). Sensitivity could not be increased further by combining more than two tests. A significant correlation was demonstrated between the level of circulating antigen and the number of parasite eggs in feces in each of the four assays. In addition, the levels of CAA and CCA in serum and urine were significantly correlated with each other. Our results indicate that diagnosis of schistosome infections by detection of circulating antigens can be significantly improved by parallel testing for multiple antigens.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 499 | 235 | 5 |
| Full Text Views | 17 | 0 | 0 |
| PDF Downloads | 17 | 0 | 0 |