Purification of Native Dengue-2 Viral Proteins and the Ability of Purified Proteins to Protect Mice

Robert Feighny Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, DC

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Jeanne Burrous Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, DC

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Jack McCown Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, DC

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Charles Hoke Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, DC

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Robert Putnak Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, DC

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DOI:
https://doi.org/10.4269/ajtmh.1992.47.405
Volume/Issue:
Volume 47: Issue 4
Page(s):
405–412
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Both the envelope structural protein and the non-structural NS1 protein have been purified from the flavivirus dengue-2 by high-pressure liquid chromatography. These purified proteins maintain their reactivity with monoclonal antibodies. When tested in mice, the envelope protein elicited neutralizing antibodies and partially protected the animals against a lethal viral challenge. The mice responded to the non-structural protein by producing antibodies; however, these antibodies were not neutralizing and the mice were not protected.

Author Notes

Copyright:
Copyright © 1992 by The American Society of Tropical Medicine and Hygiene 1992
Published Online:
Oct 1992
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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