Molecular Characterization of Mexican Stocks of Trypanosoma Cruzi using Total DNA

Jorge E. Zavala-CastroDepartment of Molecular Biology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico, Mexico

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Oscar Velasco-CastrejonDepartment of Molecular Biology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico, Mexico

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Roberto HernandezDepartment of Molecular Biology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico, Mexico

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Seventeen Mexican Trypanosoma cruzi stocks and five South American reference strains were analyzed by Hind III restriction fragment length polymorphisms associated with ribosomal RNA gene spacers and by Hinf I digestion patterns of total DNA. Our findings demonstrate the occurrence of genetic heterogeneity within these stocks. Hierarchic and non-hierarchic clustering of these molecular characters allowed the formation of groups that correlate with the geographic origin of the stocks. The Hinf I digestion pattern permitted the identification of DNA fragments from the kinetoplast maxicircles, and therefore represents a simple and convenient method for conducting epidemiologic surveys in laboratories in developing countries.

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