Molecular Characterization of Mexican Stocks of Trypanosoma Cruzi using Total DNA

Jorge E. Zavala-Castro Department of Molecular Biology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico, Mexico

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Oscar Velasco-Castrejon Department of Molecular Biology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico, Mexico

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Roberto Hernandez Department of Molecular Biology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico, Mexico

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DOI:
https://doi.org/10.4269/ajtmh.1992.47.201
Volume/Issue:
Volume 47: Issue 2
Page(s):
201–209
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Seventeen Mexican Trypanosoma cruzi stocks and five South American reference strains were analyzed by Hind III restriction fragment length polymorphisms associated with ribosomal RNA gene spacers and by Hinf I digestion patterns of total DNA. Our findings demonstrate the occurrence of genetic heterogeneity within these stocks. Hierarchic and non-hierarchic clustering of these molecular characters allowed the formation of groups that correlate with the geographic origin of the stocks. The Hinf I digestion pattern permitted the identification of DNA fragments from the kinetoplast maxicircles, and therefore represents a simple and convenient method for conducting epidemiologic surveys in laboratories in developing countries.

Author Notes

Copyright:
Copyright © 1992 by The American Society of Tropical Medicine and Hygiene 1992
Published Online:
Aug 1992
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637

Expert Committee on Hepatitis

First Report. World Health Organization Technical Report Series No. 62 (Available also in French). 26 pp. Geneva, W.H.O., Palais des Nations. March, 1953. Price 1/6, $0.20, Fr. fr. 65.-; Sw. fr. 0.80

Parasitic Infections of Man

by Harry Most, M.D., Editor. Symposium No. 4 of the Section on Microbiology, New York Academy of Medicine. 220 pp., cloth. New York, Columbia University Press, 1951. $4.50

Author:
G. F. Otto

Immunology

by Noble Pierce Sherwood. Ph.D., M.D., Professor of Bacteriology, University of Kansas—3rd Edition, 731 pp., illustrated, cloth. St. Louis: The C. V. Mosby Co., 1951. $8.00

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