Molecular Characterization of Mexican Stocks of Trypanosoma Cruzi using Total DNA

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  • Department of Molecular Biology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico, Mexico

Seventeen Mexican Trypanosoma cruzi stocks and five South American reference strains were analyzed by Hind III restriction fragment length polymorphisms associated with ribosomal RNA gene spacers and by Hinf I digestion patterns of total DNA. Our findings demonstrate the occurrence of genetic heterogeneity within these stocks. Hierarchic and non-hierarchic clustering of these molecular characters allowed the formation of groups that correlate with the geographic origin of the stocks. The Hinf I digestion pattern permitted the identification of DNA fragments from the kinetoplast maxicircles, and therefore represents a simple and convenient method for conducting epidemiologic surveys in laboratories in developing countries.