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Molecular Characterization of Mexican Stocks of Trypanosoma Cruzi using Total DNA
Jorge E. Zavala-Castro
Jorge E. Zavala-Castro
Department of Molecular Biology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico, Mexico
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Oscar Velasco-Castrejon
Oscar Velasco-Castrejon
Department of Molecular Biology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico, Mexico
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Roberto Hernandez
Roberto Hernandez
Department of Molecular Biology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico, Mexico
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Seventeen Mexican Trypanosoma cruzi stocks and five South American reference strains were analyzed by Hind III restriction fragment length polymorphisms associated with ribosomal RNA gene spacers and by Hinf I digestion patterns of total DNA. Our findings demonstrate the occurrence of genetic heterogeneity within these stocks. Hierarchic and non-hierarchic clustering of these molecular characters allowed the formation of groups that correlate with the geographic origin of the stocks. The Hinf I digestion pattern permitted the identification of DNA fragments from the kinetoplast maxicircles, and therefore represents a simple and convenient method for conducting epidemiologic surveys in laboratories in developing countries.
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| Past two years | Past Year | Past 30 Days | |
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