Construction of a Genomic DNA Library of the Toxoplasma Gondii ZS2 Strain, Screening of Specific Clones, and DNA Diagnosis of Toxoplasmosis

Ai-Di XiaDepartment of Biochemistry, Laboratory of Molecular Biology, and Department of Parasitology, Shanghai Second Medical University, Shanghai, People's Republic of China

Search for other papers by Ai-Di Xia in
Current site
Google Scholar
PubMed
Close
,
Yun-Zhong GuDepartment of Biochemistry, Laboratory of Molecular Biology, and Department of Parasitology, Shanghai Second Medical University, Shanghai, People's Republic of China

Search for other papers by Yun-Zhong Gu in
Current site
Google Scholar
PubMed
Close
,
Shi-Jie XuDepartment of Biochemistry, Laboratory of Molecular Biology, and Department of Parasitology, Shanghai Second Medical University, Shanghai, People's Republic of China

Search for other papers by Shi-Jie Xu in
Current site
Google Scholar
PubMed
Close
,
Ke-Min WangDepartment of Biochemistry, Laboratory of Molecular Biology, and Department of Parasitology, Shanghai Second Medical University, Shanghai, People's Republic of China

Search for other papers by Ke-Min Wang in
Current site
Google Scholar
PubMed
Close
,
Hui-Zhen YangDepartment of Biochemistry, Laboratory of Molecular Biology, and Department of Parasitology, Shanghai Second Medical University, Shanghai, People's Republic of China

Search for other papers by Hui-Zhen Yang in
Current site
Google Scholar
PubMed
Close
,
Ke-Ji XuDepartment of Biochemistry, Laboratory of Molecular Biology, and Department of Parasitology, Shanghai Second Medical University, Shanghai, People's Republic of China

Search for other papers by Ke-Ji Xu in
Current site
Google Scholar
PubMed
Close
,
Zong-Li QianDepartment of Biochemistry, Laboratory of Molecular Biology, and Department of Parasitology, Shanghai Second Medical University, Shanghai, People's Republic of China

Search for other papers by Zong-Li Qian in
Current site
Google Scholar
PubMed
Close
, and
Shi-Shu ChenDepartment of Biochemistry, Laboratory of Molecular Biology, and Department of Parasitology, Shanghai Second Medical University, Shanghai, People's Republic of China

Search for other papers by Shi-Shu Chen in
Current site
Google Scholar
PubMed
Close
Restricted access

We have constructed a genomic DNA library of the Toxoplasma gondii ZS2 strain and isolated a specific cloned DNA sequence from this organism. The restriction map of this cloned 1.1-kb DNA fragment was analyzed. Southern and dot-blot analyses showed that the 32P-labeled DNA fragment hybridized to parasite DNA, to DNAs from peripheral blood leukocytes and the thymus of baby pigs that were artificially infected with T. gondii, and to DNAs of T. gondii-positive anencephalic and hydrocephalic fetuses. It did not hybridize with DNA from controls, (i.e., normal human and baby pig peripheral blood leukocytes, spleen of normal mice, Plasmodium falciparum, Pneumocystis carinii, and pBR322). As few as 100 T. gondii parasites or 500 pg of purified DNA from T. gondii can be detected by dot-blot hybridization. This probe method was specific and sensitive, and has been used successfully in detecting various clinical cases of toxoplasmosis with T. gondii.

Save