Growth of Dengue Type 2 Virus Isolates in Human Peripheral Blood Leukocytes Correlates with Severe and Mild Dengue Disease

David M. MorensDepartment of Tropical Medicine and Medical Microbiology, University of Hawaii School of Medicine, Leahi Hospital, Division of Vector-Borne Infectious Diseases, Center for Infectious Diseases, Centers for Disease Control, The Rockefeller Foundation, Honolulu, Hawaii

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Nyven J. MarchetteDepartment of Tropical Medicine and Medical Microbiology, University of Hawaii School of Medicine, Leahi Hospital, Division of Vector-Borne Infectious Diseases, Center for Infectious Diseases, Centers for Disease Control, The Rockefeller Foundation, Honolulu, Hawaii

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May C. ChuDepartment of Tropical Medicine and Medical Microbiology, University of Hawaii School of Medicine, Leahi Hospital, Division of Vector-Borne Infectious Diseases, Center for Infectious Diseases, Centers for Disease Control, The Rockefeller Foundation, Honolulu, Hawaii

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Scott B. HalsteadDepartment of Tropical Medicine and Medical Microbiology, University of Hawaii School of Medicine, Leahi Hospital, Division of Vector-Borne Infectious Diseases, Center for Infectious Diseases, Centers for Disease Control, The Rockefeller Foundation, Honolulu, Hawaii

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We tested three dengue type 2 (DEN-2) isolates from children with clinically apparent but mild secondary dengue infections, and 10 isolates from children with moderately severe dengue hemorrhagic fever, and noted significant growth differences in peripheral blood leukocytes, but not in C6/36 cells. We also observed cytopathic effects in C6/36 cells that correlated with disease severity. These preliminary observations suggest the possibility that viral factors, whether surface antigens, attachment sites for entry into leukocytes, or intrinsic replication properties in human mononuclear phagocytes, might contribute to enhanced DEN infection and to the severity of the disease.

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