A Modified Agar Plate Method for Detection of Strongyloides Stercoralis

Kaori KogaDepartment of Parasitology and Research Group of Tropical Medicine, Gifu University School of Medicine, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Gifu, Japan

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Shiro KasuyaDepartment of Parasitology and Research Group of Tropical Medicine, Gifu University School of Medicine, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Gifu, Japan

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Chirasak KhamboonruangDepartment of Parasitology and Research Group of Tropical Medicine, Gifu University School of Medicine, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Gifu, Japan

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Keturat SukhavatDepartment of Parasitology and Research Group of Tropical Medicine, Gifu University School of Medicine, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Gifu, Japan

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Masatoshi IedaDepartment of Parasitology and Research Group of Tropical Medicine, Gifu University School of Medicine, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Gifu, Japan

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Naoyoshi TakatsukaDepartment of Parasitology and Research Group of Tropical Medicine, Gifu University School of Medicine, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Gifu, Japan

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Kenji KitaDepartment of Parasitology and Research Group of Tropical Medicine, Gifu University School of Medicine, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Gifu, Japan

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Hiroshi OhtomoDepartment of Parasitology and Research Group of Tropical Medicine, Gifu University School of Medicine, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Gifu, Japan

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The agar plate method is a new technique with high detection rates for coprological diagnosis of human stronglyoidiasis. This report details modifications of the technique and establishes a standardized procedure. We recommend that all plates should be carefully observed using a microscope because macroscopic observation can lead to false negative results. It is also advisable to pour formalin solution directly into microscopically positive dishes to collect worms by sedimentation. This procedure enables one to observe worms otherwise hidden. Sealing dishes with adhesive tape prevents larvae from crawling out of the dishes, eliminating any possibility in the reduction of detection rates, and greatly improves the safety conditions for the technician performing the procedure. We consider the agar plate method to be superior to the filter paper method in detecting Strongyloides, and we believe that it will eventually become the technique of choice.

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