Inhibitory Activity against Plasmodium Vivax Sporozoites Induced by Plasma from Saimiri Monkeys Immunized with Circumsporozoite Recombinant Proteins or Irradiated Sporozoites

Pascal Millet Division of Parasitic Diseases, and Scientific Resources Program, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, US Department of Health and Human Services, Chiron Corporation, Department of Medical and Molecular Parasitology, New York University School of Medicine, Atlanta, Georgia

Search for other papers by Pascal Millet in
Current site
Google Scholar
PubMed Close
,
William E. Collins Division of Parasitic Diseases, and Scientific Resources Program, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, US Department of Health and Human Services, Chiron Corporation, Department of Medical and Molecular Parasitology, New York University School of Medicine, Atlanta, Georgia

Search for other papers by William E. Collins in
Current site
Google Scholar
PubMed Close
,
J. Roger Broderson Division of Parasitic Diseases, and Scientific Resources Program, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, US Department of Health and Human Services, Chiron Corporation, Department of Medical and Molecular Parasitology, New York University School of Medicine, Atlanta, Georgia

Search for other papers by J. Roger Broderson in
Current site
Google Scholar
PubMed Close
,
Ian Bathurst Division of Parasitic Diseases, and Scientific Resources Program, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, US Department of Health and Human Services, Chiron Corporation, Department of Medical and Molecular Parasitology, New York University School of Medicine, Atlanta, Georgia

Search for other papers by Ian Bathurst in
Current site
Google Scholar
PubMed Close
,
Elisabeth H. Nardin Division of Parasitic Diseases, and Scientific Resources Program, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, US Department of Health and Human Services, Chiron Corporation, Department of Medical and Molecular Parasitology, New York University School of Medicine, Atlanta, Georgia

Search for other papers by Elisabeth H. Nardin in
Current site
Google Scholar
PubMed Close
, and
Ruth S. Nussenzweig Division of Parasitic Diseases, and Scientific Resources Program, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, US Department of Health and Human Services, Chiron Corporation, Department of Medical and Molecular Parasitology, New York University School of Medicine, Atlanta, Georgia

Search for other papers by Ruth S. Nussenzweig in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.4269/ajtmh.1991.45.44
Volume/Issue:
Volume 45: Issue 1
Page(s):
44–48
Restricted access
Get Permissions

Two Saimiri monkey vaccine trials have been conducted comparing four recombinant Plasmodium vivax circumsporozoite proteins and irradiated sporozoites. Only a small number of animals immunized with certain recombinants or irradiated sporozoites became fully protected against sporozoite challenge. Preimmunization and postimmunization plasma samples obtained from 30 monkeys on the day of challenge were tested in an in vitro assay based on sporozoite development into exoerythrocytic stages in primary cultures of Saimiri monkey hepatocytes. The percentage of inhibition was determined by comparison of the number of exoerythrocytic stages developing from sporozoites preincubated with a preimmunization and a postimmunization plasma sample of each animal. The plasma samples of the day of challenge of nearly all the immunized animals had a variable, but significant inhibitory effect, when compared with the corresponding preimmunization sample. We found no correlation between the degree of in vitro inhibition of liver stage development, and the in vivo protection against sporozoite challenge of individual animals. The variable results of the incubation of sporozoites with “normal” plasma of different animals indicates that the in vitro results were affected by plasma factors unrelated to anti-sporozoite antibodies.
Copyright:
Copyright © 1991 by The American Society of Tropical Medicine and Hygiene 1991
Published Online:
Jul 1991
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Past two years Past Year Past 30 Days
Abstract Views 237 77 5
Full Text Views 13 0 0
PDF Downloads 12 0 0
 
Membership Banner
 
 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save