Schistosoma Mansoni Tropomyosin: Production and Purification of the Recombinant Protein and Studies on its Immunodiagnostic Potential

Han Xu Departments of Microbiology, Pathology, and Biochemistry, School of Medicine, State University of New York, Department of Epidemiology, School of Public Health, The University of Michigan, Buffalo, New York

Search for other papers by Han Xu in
Current site
Google Scholar
PubMed Close
,
David M. Rekosh Departments of Microbiology, Pathology, and Biochemistry, School of Medicine, State University of New York, Department of Epidemiology, School of Public Health, The University of Michigan, Buffalo, New York

Search for other papers by David M. Rekosh in
Current site
Google Scholar
PubMed Close
,
William Andrews Departments of Microbiology, Pathology, and Biochemistry, School of Medicine, State University of New York, Department of Epidemiology, School of Public Health, The University of Michigan, Buffalo, New York

Search for other papers by William Andrews in
Current site
Google Scholar
PubMed Close
,
Gene I. Higashi Departments of Microbiology, Pathology, and Biochemistry, School of Medicine, State University of New York, Department of Epidemiology, School of Public Health, The University of Michigan, Buffalo, New York

Search for other papers by Gene I. Higashi in
Current site
Google Scholar
PubMed Close
,
Laura Nicholson Departments of Microbiology, Pathology, and Biochemistry, School of Medicine, State University of New York, Department of Epidemiology, School of Public Health, The University of Michigan, Buffalo, New York

Search for other papers by Laura Nicholson in
Current site
Google Scholar
PubMed Close
, and
Philip T. Loverde Departments of Microbiology, Pathology, and Biochemistry, School of Medicine, State University of New York, Department of Epidemiology, School of Public Health, The University of Michigan, Buffalo, New York

Search for other papers by Philip T. Loverde in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.4269/ajtmh.1991.45.121
Volume/Issue:
Volume 45: Issue 1
Page(s):
121–131
Restricted access
Get Permissions

A cDNA that encodes Schistosoma mansoni tropomyosin, except for 10 amino acids at the amino terminus, has been cloned into a pOTSNCO plasmid vector. Induced expression resulted in a constant level of recombinant protein production. The recombinant S. mansoni tropomyosin was purified from preparative SDS-PAGE gel and by a combination of 20% ammonium sulfate fractionation and fast protein liquid chromatographyionexchange chromatography. The purified recombinant S. mansoni tropomyosin was tested as an immunodiagnostic reagent in Western blot and enzyme-linked immunosorbent assays. Sera from individual patients with chronic S. mansoni infection, but not S. haematobium, S. japonicum, parasitic infections other than schistosomiasis, and without infection reacted with the recombinant tropomyosin. The species specificity of S. mansoni tropomyosin suggests that further study of its potential as an immunodiagnostic reagent is warranted.

Author Notes

Copyright:
Copyright © 1991 by The American Society of Tropical Medicine and Hygiene 1991
Published Online:
Jul 1991
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Past two years Past Year Past 30 Days
Abstract Views 607 310 7
Full Text Views 13 2 0
PDF Downloads 15 3 0
 
 
 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save