Flavivirus Type-Specific Antigens Produced from Fusions of a Portion of the E Protein Gene with the Escherichia coli trpe Gene

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  • Yale University School of Medicine, Yale Arbovirus Research Unit, Department of Epidemiology and Public Health, Instituto Adolfo Lutz, Departamento de Virologia, Secao de Arbovirus, Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, New Haven, CT, USA
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Based on previous data showing that JEV and DEN-1 fusion proteins can be used as antigens, we engineered analogous fusion proteins for the same regions of the E proteins of DEN-2, DEN-3, and DEN-4, using the polymerase chain reaction. The resulting fusion proteins were tested in a modified ELISA for their reactivity with mouse immune ascitic fluids (MIAF) generated against 10 different flaviviruses. The results showed that these recombinant antigens could be used as type-specific immunological reagents, and suggest that these antigens could serve as the basis for rapid, safe, and inexpensive flavivirus serosurveys.