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Isolation of Dengue Virus with a Human Promonocyte Cell Line
Wu-Tse Liu
Wu-Tse Liu
School of Medical Technology, National Yang-Ming Medical College, Graduate Institute of Medicine and School of Public Health, Kaohsiung Medical College, Taipei, Taiwan, 11221, Republic of China
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Chich-Liang Chen
Chich-Liang Chen
School of Medical Technology, National Yang-Ming Medical College, Graduate Institute of Medicine and School of Public Health, Kaohsiung Medical College, Taipei, Taiwan, 11221, Republic of China
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Susan Shin-Jung Lee
Susan Shin-Jung Lee
School of Medical Technology, National Yang-Ming Medical College, Graduate Institute of Medicine and School of Public Health, Kaohsiung Medical College, Taipei, Taiwan, 11221, Republic of China
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Ch'ien-Chen Chan
Ch'ien-Chen Chan
School of Medical Technology, National Yang-Ming Medical College, Graduate Institute of Medicine and School of Public Health, Kaohsiung Medical College, Taipei, Taiwan, 11221, Republic of China
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Fu-Luen Lo
Fu-Luen Lo
School of Medical Technology, National Yang-Ming Medical College, Graduate Institute of Medicine and School of Public Health, Kaohsiung Medical College, Taipei, Taiwan, 11221, Republic of China
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Ying-Chin Ko
Ying-Chin Ko
School of Medical Technology, National Yang-Ming Medical College, Graduate Institute of Medicine and School of Public Health, Kaohsiung Medical College, Taipei, Taiwan, 11221, Republic of China
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In October–November, 1988 there was an outbreak of dengue fever in the Kaoshiung area of southern Taiwan. We collected 100 serum samples from 96 patients at the onset of their fever for virus cultures and identification. A human promonocyte cell line (HL-CZ) established in our laboratory was used and proved to be susceptible for dengue virus propagation. Type 1 dengue virus in the HL-CZ cell culture was identified by immunofluorescence tests using monoclonal antibodies, and also by hemagglutination tests with goose red blood cells. The density of the virus particles, as measured by sucrose gradient ultracentrifugation, ranged from 1.186 to 1.224 g/ml. The virus yield from this cell culture is comparable with that from the C6/36 mosquito cell line. There was a significant correlation between the antibody responses tested with Western dot blots and hemagglutination inhibition techniques.
| Past two years | Past Year | Past 30 Days | |
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