Acridine Orange Detection of Plasmodium Falciparum Malaria: Relationship between Sensitivity and Optical Configuration

Gary W. Long Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland

Search for other papers by Gary W. Long in
Current site
Google Scholar
PubMed Close
,
Trevor R. Jones Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland

Search for other papers by Trevor R. Jones in
Current site
Google Scholar
PubMed Close
,
Leland S. Rickman Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland

Search for other papers by Leland S. Rickman in
Current site
Google Scholar
PubMed Close
,
Roy Trimmer Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland

Search for other papers by Roy Trimmer in
Current site
Google Scholar
PubMed Close
, and
Stephen L. Hoffman Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland

Search for other papers by Stephen L. Hoffman in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.4269/ajtmh.1991.44.402
Volume/Issue:
Volume 44: Issue 4
Page(s):
402–405
Restricted access
Get Permissions

Blood samples collected from five volunteers participating in a P. falciparum infectivity trial were examined to determine the efficacy of the acridine orange technique. Several lens configurations were tested for efficiency in the diagnosis of malaria using this system. There was no significant difference in the sensitivity for detecting positive specimens or number of parasites among three lens configurations: a 50 × long working distance objective (0.34mm) with either a 10 × ocular (total magnification 500 ×) or a 12.5 × ocular (625 ×) and a 750 × configuration using a 50 × objective with a shorter working distance (0.24mm). All three lens configurations were significantly better than the 1,000 × configuration using a commonly available 100 × oil immersion objective. The results achieved using this lens still exceeded the sensitivity of the thick blood film.

Author Notes

Copyright:
Copyright © 1991 by The American Society of Tropical Medicine and Hygiene 1991
Published Online:
Apr 1991
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Past two years Past Year Past 30 Days
Abstract Views 1098 718 47
Full Text Views 15 0 0
PDF Downloads 13 0 0
 

 

 

 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save