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Automated Flow Cytometric Analysis of Drug Susceptibility of Malaria Parasites
Philip H. Van Vianen
Philip H. Van Vianen
University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand
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Sodsri Thaithong
Sodsri Thaithong
University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand
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Paul P. Reinders
Paul P. Reinders
University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand
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Anneloes Van Engen
Anneloes Van Engen
University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand
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Maarten Van Der Keur
Maarten Van Der Keur
University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand
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Hans J. Tanke
Hans J. Tanke
University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand
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Hugo J. Van Der Kaay
Hugo J. Van Der Kaay
University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand
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Barend Mons
Barend Mons
University of Leiden, The Netherlands; Chulalongkorn University, Bangkok, Thailand
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A method is described for the fully automated reading of Plasmodium falciparum drug susceptibility tests. Cultured material was fixed and could be stored for ≥6 months until analysis. The parasites were stained for DNA with the fluorescent dye Hoechst 33258 and analyzed by flow cytometry. The procedure was done in 96-well microtiter plates, after which the material was directed through the sensing region in the flow cytometer. The data resulting from the analysis were stored by microcomputer and processed by a program developed for this purpose. Using this method, a number of different parameters describing the growth in culture can be assessed.
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| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 776 | 481 | 28 |
| Full Text Views | 17 | 1 | 0 |
| PDF Downloads | 19 | 2 | 0 |