Preparation of an Attenuated Dengue 4 (341750 Carib) Virus Vaccine

I. Pre-Clinical Studies

Nyven J. MarchetteUniversity of Hawaii, Walter Reed Army Institute of Research, University of Puerto Rico, Center for Disease Control, San Juan Laboratories, Honolulu, Hawaii, Puerto Rico

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Doria R. DuboisUniversity of Hawaii, Walter Reed Army Institute of Research, University of Puerto Rico, Center for Disease Control, San Juan Laboratories, Honolulu, Hawaii, Puerto Rico

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Linda K. LarsenUniversity of Hawaii, Walter Reed Army Institute of Research, University of Puerto Rico, Center for Disease Control, San Juan Laboratories, Honolulu, Hawaii, Puerto Rico

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Peter L. SummersUniversity of Hawaii, Walter Reed Army Institute of Research, University of Puerto Rico, Center for Disease Control, San Juan Laboratories, Honolulu, Hawaii, Puerto Rico

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Edmundo G. KraiselburdUniversity of Hawaii, Walter Reed Army Institute of Research, University of Puerto Rico, Center for Disease Control, San Juan Laboratories, Honolulu, Hawaii, Puerto Rico

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Duane J. GublerUniversity of Hawaii, Walter Reed Army Institute of Research, University of Puerto Rico, Center for Disease Control, San Juan Laboratories, Honolulu, Hawaii, Puerto Rico

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Kenneth H. EckelsUniversity of Hawaii, Walter Reed Army Institute of Research, University of Puerto Rico, Center for Disease Control, San Juan Laboratories, Honolulu, Hawaii, Puerto Rico

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Dengue 4 (DEN-4) virus strain 341750 Carib was modified by serial passage in primary canine kidney (PCK) cell cultures. By the 15th PCK passage, this virus was less infectious for monkeys and resulted in a significantly reduced viremia as compared to the parent DEN-4 virus. The 30th PCK passage of DEN-4 341750 Carib was non-infectious for monkeys. A vaccine prepared at the 20th PCK passage in DBS-FRhL-2 cells stimulated the production of both neutralizing and hemagglutination inhibition antibodies in monkeys; these animals were also protected against challenge with the homologous strain as well as a heterologous strain of DEN-4. An ID50 titration in monkeys resulted in a titer of > 104 plaque-forming units (PFU) for the vaccine virus and 0.5 PFU for the parent virus. Reduced monkey infectivity of this magnitude has been correlated with human attenuation in previous dengue vaccine candidates. The DEN-4 strain 341750 Carib PCK-20/FRhL-4 vaccine has been characterized and sufficiently tested to be considered for safety and immunogenicity trials in humans.

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