Variability of Trypanosoma cruzi Epimastigote Surface Antigens with Changes in the Temperature of the Cultures

José A. O'Daly Instituto Venezolano de Investigaciones Científicas, Centre of Microbiology and Cell Biology, Caracas, Venezuela

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Nina Polanco Instituto Venezolano de Investigaciones Científicas, Centre of Microbiology and Cell Biology, Caracas, Venezuela

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Trypanosoma cruzi epimastigotes changed their pattern of surface proteins when the temperature of growth rose from 30°C to 34°C. Challenge of mice with blood-form trypomastigotes produced high parasitemias when animals were immunized with surface proteins from epimastigotes cultured at 30°C and with Nonidet P-40-extracted epimastigotes pellets cultured at 34°C. However, low parasitemias were recorded after immunization with surface proteins from epimastigotes cultured at 34°C or Nonidet P-40-extracted epimastigotes pellets at 30°C. The lowest parasitemia, together with the longest survival time and absence of immunosuppression, was observed after immunization of mice with the product extracted with non-ionic detergent from epimastigotes grown at 30°C and treated with tosyl-L-lysine-chloromethyl ketone.

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