Cultivation of Sexually Mature Brugia malayi In Vitro

W. A. RiberuU.S. Naval Medical Research Unit No. 2, Jakarta Detachment, Jakarta, Indonesia

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Soeroto AtmosoedjonoU.S. Naval Medical Research Unit No. 2, Jakarta Detachment, Jakarta, Indonesia

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PurnomoU.S. Naval Medical Research Unit No. 2, Jakarta Detachment, Jakarta, Indonesia

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S. TirtokusumoU.S. Naval Medical Research Unit No. 2, Jakarta Detachment, Jakarta, Indonesia

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M. J. BangsU.S. Naval Medical Research Unit No. 2, Jakarta Detachment, Jakarta, Indonesia

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J. K. BairdU.S. Naval Medical Research Unit No. 2, Jakarta Detachment, Jakarta, Indonesia

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Sexually mature male and female Brugia malayi were developed from third stage larvae after 60 days in the in vitro culture system described by Franke and others in 1987 (Am J Trop Med Hyg 37: 370–375). Between 75 and 100 days in culture, many worms produced living microfilariae. Each gravid female produced 200–1,500 microfilariae/day.

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